Analysis Tools
mortality/aging
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• at 750 cGy, all exposed mice die compared to only 2 of 23 similarly treated wild-type mice
• however, mice reconstituted with wild-type bone marrow exhibit improved survival, and radiation sensitivity of thymocytes and E14.5 mouse embryonic fibroblasts is normal
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• mice are present at normal Mendelian ratios at E18.5 but exhibit lethality during the first day after birth
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• only 40% of expected embryos survive to weaning with mice dieing within the first few days of birth
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reproductive system
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• mice lack mature sperm and instead exhibit spermatozoa with round heads
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hematopoietic system
small thymus
(
J:145579
)
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• thymocytes are reduced 30% compared to in wild-type mice
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• cells implanted into Rag2 and Il2rg null mice exhibit defects in B and T cell development unlike wild-type cells
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• erythro-myeloid progenitor cells (Lin-Kit+Sca1-) bone marrow cells are reduced 40% compared to in wild-type mice
• megakaryocyte-erythrocyte progenitor cells are decreased compared to in wild-type mice
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• granulocyte-monocyte progenitors are decreased compared to in wild-type mice
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• thymocytes are reduced 30% compared to in wild-type mice
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• DN1 cells are reduced 7-fold, DN2 3.6-fold, and the earliest thymocyte progenitor cells 16.9-fold compared to in wild-type mice
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• Lin-Sca+Kit+ (LSK) cells are decreased 2-fold compared to in wild-type mice
• the total number of long-term hematopoietic stem cells (HSCs; CD34-Flt3-) is decreased 2-fold and the lymphoid-primed multipotent progenitors 4-fold compared to in wild-type mice
• the number of short term HSC (LSK CD34+Flt3-) is decreased 25% compared to in wild-type mice despite a 3-fold increase in cell cycling
• Lin- bone marrow cells are unable to rescue lethally irradiated mice unlike wild-type cells
• however, homing of Lin- bone marrow cells in irradiated wild-type mice is normal
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small spleen
(
J:145579
)
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• splenocytes are reduced 30% compared to in wild-type mice
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growth/size/body
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• at 6 weeks
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• at 6 weeks of age, body weight is reduced compared to normal by 16% for males and 12% for females
• mice exhibit decreased body weight during pubertal growth and adulthood
• however, weight at E18.5 is normal
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immune system
| N |
• despite decreased T cell numbers, VDJ recombination is normal
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small thymus
(
J:145579
)
|
• thymocytes are reduced 30% compared to in wild-type mice
|
|
• cells implanted into Rag2 and Il2rg null mice exhibit defects in B and T cell development unlike wild-type cells
|
|
• thymocytes are reduced 30% compared to in wild-type mice
|
|
• DN1 cells are reduced 7-fold, DN2 3.6-fold, and the earliest thymocyte progenitor cells 16.9-fold compared to in wild-type mice
|
small spleen
(
J:145579
)
|
• splenocytes are reduced 30% compared to in wild-type mice
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homeostasis/metabolism
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• at 750 cGy, all exposed mice die compared to only 2 of 23 similarly treated wild-type mice
• however, mice reconstituted with wild-type bone marrow exhibit improved survival, and radiation sensitivity of thymocytes and E14.5 mouse embryonic fibroblasts is normal
|
endocrine/exocrine glands
small thymus
(
J:145579
)
|
• thymocytes are reduced 30% compared to in wild-type mice
|
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• thymocytes are reduced 30% compared to in wild-type mice
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cellular
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• mice lack mature sperm and instead exhibit spermatozoa with round heads
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