Phenotypes Associated with This Genotype

Genotype
MGI:3835203

• Allelic Composition: Itgb1^tm1Lscd/Itgb1^tm1Lscd
• Genetic Background: involves: 129X1/SvJ * Black Swiss

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:28266 )

• lethality occurs between E4.5 and E5.5 due to inner cell mass defects

embryo

abnormal blastocoele morphology ( J:28266 )

• E4.5 embryos have collapsed blastocoeles

abnormal inner cell mass morphology ( J:28266 )

• isolated inner cell masses attach, spread and differentiate very poorly on a fibronectin substrate

inner cell mass degeneration ( J:28266 )

• inner cell mass of cultured blastocytes have highly restricted growth and defective endoderm morphogenesis and migration

• 48 hours of blastocyst culture in serum leads to inner cell masses that are significantly smaller and appear disorganized compared to controls

abnormal trophoblast layer morphology ( J:28266 )

• the trophoblasts of E4.5 embryos fails to extensively invade the maternal decidua

• trophoblast outgrowth from cultured blastocytes is defective when laminin is provided as a substrate

abnormal visceral endoderm morphology ( J:28266 )

• the monolayer of visceral endoderm is not formed in these inner cell masses despite the presence of laminin-positive cell clusters

• only 3 of 17 inner masses demonstrate any morphogenesis of the endoderm under this conditions compared to the 100% morphogenesis that occurs to controls