Phenotypes Associated with This Genotype

Genotype
MGI:3834573

• Allelic Composition: Cd14^tm1Smg/Cd14^tm1Smg
• Genetic Background: C.129S1-CD14^tm1Smg

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal neutrophil physiology ( J:127072 )

• increased numbers of neutrophils migrate to the peritoneal cavity after i.p. infection with E. coli or after injection with LPS

• mice have about 5-fold more neutrophils in the peritoneal cavity than controls during the first 5 hour post-injection

• detectable amounts of neutrophils are present within 3 hours of LPS injection while neutrophils are not observed in wild-type controls until 24 hours after LPS injection

• neutrophil response can be induced with very low amounts of LPS

abnormal macrophage physiology ( J:116691 )

• macrophages are less responsive to LPS compared to controls as determined by TNF secretion

• macrophages are least responsive to carbohydrated (i.e. smooth) forms of LPS where 150,000 fold more is needed to generate the same response as in controls

• in contrast, macrophages are only 4-fold less responsive to the non-carbohydrated form of LPS known as lipid A

decreased tumor necrosis factor secretion ( J:116691 )

• macrophages produce less TNF in response LPS than in controls

decreased susceptibility to bacterial infection ( J:127072 )

• eight hours after infection with E. coli, mice have 10-fold lower bacterial counts in the blood as do infection wild-type controls

• the bacterial resistance is abolished when mice are made neutropenic by cyclophosphamide treatment

hematopoietic system

abnormal neutrophil physiology ( J:127072 )

• increased numbers of neutrophils migrate to the peritoneal cavity after i.p. infection with E. coli or after injection with LPS

• mice have about 5-fold more neutrophils in the peritoneal cavity than controls during the first 5 hour post-injection

• detectable amounts of neutrophils are present within 3 hours of LPS injection while neutrophils are not observed in wild-type controls until 24 hours after LPS injection

• neutrophil response can be induced with very low amounts of LPS

abnormal macrophage physiology ( J:116691 )

• macrophages are less responsive to LPS compared to controls as determined by TNF secretion

• macrophages are least responsive to carbohydrated (i.e. smooth) forms of LPS where 150,000 fold more is needed to generate the same response as in controls

• in contrast, macrophages are only 4-fold less responsive to the non-carbohydrated form of LPS known as lipid A