Analysis Tools
immune system
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• bone marrow derived macrophages produce less IL-6 and TNF when cultured in the presence of L. monocytogenes
• in macrophages pre-treated with TLR ligands (i.e. "tolerized"), macrophages produce significantly less inflammatory cytokines upon incubation with L. monocytogenes than controls that were LPS pre-treated
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• bone marrow derived macrophages secrete 40% the amount of IL-6 as wild-type controls due in response to culturing with L. monocytogenes
• IL-6 secretion is 7% that of controls when macrophages are pre-treated with LPS prior to L. monocytogenes incubation
• IL-6 secretion is 18% that of controls when macrophages are pre-treated with E. Coli prior to L. monocytogenes incubation
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• bone marrow derived macrophages secrete 56% the amount of TNF as wild-type controls in response to culturing with L. monocytogenes
• TNF secretion is 13% that of controls when macrophages are pre-treated with LPS prior to L. monocytogenes incubation
• TNF secretion is 26% that of controls when macrophages are pre-treated with E. Coli prior to L. monocytogenes incubation
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• there is an approximately 5-fold higher number of bacterial colony-forming units in the liver and spleen than in wild-type mice 2 days after infection with L. monocytogenes
• when mice are pre-treated with LPS before infection, bacterial load in the liver and spleen is about 50- to 70- fold higher than in wild-type mice
• mutant mice that are pretreated with LPS before L. monocytogenes infection have a survival rate of about 35% compared to controls that have a 100% survival rate
• these LPS pre-treated mice have a higher number of liver microabcesses than controls after L. monocytogenes infection that were LPS pre-treated
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hematopoietic system
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• bone marrow derived macrophages produce less IL-6 and TNF when cultured in the presence of L. monocytogenes
• in macrophages pre-treated with TLR ligands (i.e. "tolerized"), macrophages produce significantly less inflammatory cytokines upon incubation with L. monocytogenes than controls that were LPS pre-treated
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