Analysis Tools
hematopoietic system
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• the number of total NK T cell numbers are normal but the majority of NK T cells is shifted from expressing a Valpha14 to Valpha19 TCR-alpha chain
• these transgenic TCR-alpha chain associate with TCR-beta V6 or V8 chains 60-70% of the time compared to 'conventional T cells' that associate with the above beta chains about 40% of the time
• nave transgenic NK T cells display a memory phenotype with more surface expression of the costimulatory molecules CD278, CD86, CD154, CD44 and CD28 than do naive splenic T cells
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immune system
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• the number of total NK T cell numbers are normal but the majority of NK T cells is shifted from expressing a Valpha14 to Valpha19 TCR-alpha chain
• these transgenic TCR-alpha chain associate with TCR-beta V6 or V8 chains 60-70% of the time compared to 'conventional T cells' that associate with the above beta chains about 40% of the time
• nave transgenic NK T cells display a memory phenotype with more surface expression of the costimulatory molecules CD278, CD86, CD154, CD44 and CD28 than do naive splenic T cells
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• transgenic NK T cells isolated from these mice induce IL-10 secretion from CD19+ B cells and other NK T cells
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• the progression and development of MOG-induced EAE is suppressed in these mice compared to both wild-type and Cd1d1/Cd1d2tm1Crw homozygotes
• mean severity score is 1.3 versus 3.3 in wild-type controls
• mice at day 15 post induction have 3-fold less infiltrating mononuclear cells in their spinal cord with a lower percentage of CD4+ T cells making up the infiltrate (6% of infiltrating cells vs 21% in littermate controls)
• memory T cells isolated from mice 10 days after disease induction produce less inflammatory cytokines (IFN-g, TNF, IL-2 and IL-17) and more IL-10
• mean day of onset is 14.3 compared to 13.6 for wild-type controls
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