Phenotypes for Tg(Igh-6-Cd80)1Gjf MGI:3823088 MGI Mouse

insulitis ( J:131077 )

• mononuclear infiltration is present, but reduced, in pancreatic islets as compared to control

• Background Sensitivity: reduction in percentage of circulating B cells beginning at 2 weeks of age

• treatment with anti-Cd8 mAb beginning at 3 weeks of age results in an increase in the percentage of B cells to almost control levels

• B cells represent 2-3% of cells in blood in contrast to 20-30% found in NOD controls

• 2 - 2.5 fold reduction of splenic B cells as compared to NOD controls

• immature/mature (B220+, IgM+) B cells reduced more than 10 fold in bone marrow

decreased immature B cell number ( J:131077 )

• immature/mature (B220+, IgM+) B cells are reduced more than 10 fold in bone marrow

decreased mature B cell number ( J:131077 )

decreased B-2 B cell number ( J:131077 )

• percentage of B-2 B cells is decreased greater than 5 fold in the peritoneal cavity, however, numbers of B-1 B cells are not significantly changed

decreased follicular B cell number ( J:131077 )

• severe depletion of mature (CD2 1ow IgM low) follicular B cells in spleen, however, marginal zone B cells are mostly unaffected

abnormal B cell activation ( J:131077 )

• splenic transgenic B cells proliferate in cell transfer experiments

decreased immunoglobulin level ( J:131077 )

• circulating IgM, IgG2b, IgG3 and IgG1 levels reduced

• treatment with anti-Cd8 mAb beginning at 3 weeks of age results in an increase in circulating immunoglobulin levels

decreased IgG1 level ( J:131077 )

decreased IgG2b level ( J:131077 )

decreased IgG3 level ( J:131077 )

decreased IgM level ( J:131077 )

abnormal CD8-positive, alpha-beta T cell physiology ( J:131077 )

• CD8+ T cell depletion leads to restoration of B cell numbers

abnormal level of surface class II molecules ( J:131077 )

• class II expression is increased by 2 fold in splenic B cells

decreased susceptibility to autoimmune diabetes ( J:131077 )

• transgenic mice do not develop diabetes

abnormal bone marrow cell number ( J:131077 )

• decrease in number of B cells in bone marrow

• pre/pro (B220+, IgM-) B cells are reduced less than 2 fold

• immature/mature (B220+, IgM+) B cells are reduced more than 10 fold

decreased B cell number ( J:131077 )