Phenotypes Associated with This Genotype

Genotype
MGI:3804724

• Allelic Composition: Cd200r1^tm1.1Rmgo/Cd200r1^tm1.1Rmgo
• Genetic Background: C57BL/6-Cd200r1^tm1.1Rmgo

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal professional antigen presenting cell physiology ( J:135069 )

• mutant antigen presenting cells in mixed lymphocyte culture fail to suppress CTL-mediated lysis of allogenic cells in the presence of a soluble form of CD200 as occurs with wild-type APCs

• APC produce more IFN-gamma and less IL-4 in the presence of soluble CD200 in these mixed lymphocyte cultures

abnormal dendritic cell physiology ( J:134313 )

• mutant bone marrow derived dendritic cells become polarized in vitro to induce antigen specific T regulatory cells when cultured with soluble CD200

• in vivo infusion of soluble CD200 for 4 weeks also leads to polarization of dendritic cells to induce T regulatory cells

• 4 x 10⁶ dendritic cells isolated from mutant mice that received soluble CD200 for 4 weeks are able to significantly prolong allogenic skin graft survival when transferred to C57BL/6 hosts

• these tolerogenic dendritic cells no longer suppress skin graft rejection if cells are cultured with LPS or a mixture of TNF and IFN-gamma before being transfered into allograft hosts

increased interleukin-12b secretion ( J:135069 )

• splenocytes cultured for 24 hours in the absence of any stimulus produce significantly more IL-12p40 than wild-type splenocytes

• IL-12p40 secretion remains significantly higher by splenocytes in the presence of LPS-stimulation

• LPS-induced IL-12p40 production by splenocytes is not suppressed by a soluble form of CD200 compared to controls where there is a 65% suppression

increased tumor necrosis factor secretion ( J:135069 )

• splenocytes cultured for 24 hours in the absence of any stimulus produce significantly more TNF than wild-type splenocytes

• TNF secretion remains significantly higher by splenocytes in the presence of LPS-stimulation

• LPS-induced TNF production by splenocytes is not suppressed by a soluble form of CD200 compared to controls where there is a 65% suppression

abnormal response to transplant ( J:135069 )

• a soluble form of CD200 fails to prolong skin allograft survival in mutant recipient mice as it does in wild-type recipient mice