Phenotypes Associated with This Genotype

Genotype
MGI:3800237

• Allelic Composition: Rnf8^{Gt(RRR260)Byg}/Rnf8^{Gt(RRR260)Byg}
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Impaired spermatogenesis in Rnf8^{Gt(AS0574)Wtsi}/Rnf8^{Gt(AS0574)Wtsi} and Rnf8^{Gt(RRR260)Byg}/Rnf8^{Gt(RRR260)Byg} males

mortality/aging

increased mortality induced by gamma-irradiation ( J:161240 )

• only 10% of mice treated with 8 Gy of gamma radiation are viable compared with 60% of similarly treated wild-type mice

premature death ( J:161240 )

• only 56% of mice are viable at 465 days compared with 97% of wild-type mice

• some mice severely runted mice die prematurely without signs of tumors

reproductive system

abnormal spermatogonia morphology ( J:161240 )

♂ • few spermatogonia are found within the seminiferous epithelium unlike in wild-type mice

abnormal seminiferous tubule epithelium morphology ( J:161240 )

♂ • disorganized

♂ • few spermatogonia are found within the seminiferous epithelium unlike in wild-type mice

seminiferous tubule degeneration ( J:161240 )

♂ • in severely affected mice without inflammation

small testis ( J:161240 )

♂

abnormal spermatogenesis ( J:161240 )

♂ • at 1.5 to 3.5 months of age

azoospermia ( J:161240 )

♂ • seminifrous tubules few to no mature spermatozoa unlike in wild-type mice

oligozoospermia ( J:161240 )

♂ • seminifrous tubules few to no mature spermatozoa unlike in wild-type mice

abnormal spermatocyte morphology ( J:161240 )

♂

male infertility ( J:161240 )

♂

immune system

increased thymocyte apoptosis ( J:161240 )

• thymocytes exhibit increased radiosensitivity compared with similarly treated wild-type cells

abnormal class switch recombination ( J:161240 )

• class switch recombination is impaired compared to in wild-type mice

decreased pro-B cell number ( J:161240 )

decreased B cell number ( J:161240 )

decreased immature B cell number ( J:161240 )

decreased pre-B cell number ( J:161240 )

decreased T cell number ( J:161240 )

decreased thymocyte number ( J:161240 )

decreased double-negative T cell number ( J:161240 )

decreased double-positive T cell number ( J:161240 )

decreased CD4-positive, alpha-beta T cell number ( J:161240 )

decreased CD8-positive, alpha-beta T cell number ( J:161240 )

decreased splenocyte number ( J:161240 )

decreased IgG1 level ( J:161240 )

decreased IgG2b level ( J:161240 )

decreased IgG3 level ( J:161240 )

neoplasm

increased mammary adenocarcinoma incidence ( J:161240 )

• in 1 mouse

increased skin tumor incidence ( J:161240 )

• in 1 mouse

increased lymphoma incidence ( J:161240 )

• in 4 mice

increased T cell derived lymphoma incidence ( J:161240 )

• in 3 mice

increased sarcoma incidence ( J:161240 )

• in 1 mouse

cellular

azoospermia ( J:161240 )

♂ • seminifrous tubules few to no mature spermatozoa unlike in wild-type mice

oligozoospermia ( J:161240 )

♂ • seminifrous tubules few to no mature spermatozoa unlike in wild-type mice

abnormal spermatocyte morphology ( J:161240 )

♂

abnormal spermatogonia morphology ( J:161240 )

♂ • few spermatogonia are found within the seminiferous epithelium unlike in wild-type mice

increased cellular sensitivity to gamma-irradiation ( J:161240 )

• bone marrow cells exhibit increased sensitivity to gamma irradiation compared with similarly treated wild-type cells

increased thymocyte apoptosis ( J:161240 )

• thymocytes exhibit increased radiosensitivity compared with similarly treated wild-type cells

decreased fibroblast proliferation ( J:161240 )

• mouse embryonic fibroblasts exhibit decreased cell proliferation compared with wild-type cells

• however, proliferation of mouse embryonic fibroblasts is rescued by hypoxic conditions

induced chromosome breakage ( J:161240 )

• following irradiation, B cells exhibit chromosome structural aberrations compared with similarly treated wild-type cells

spontaneous chromosome breakage ( J:161240 )

• chromosome breaks and metaphase aberrations in activated B cells are increased 4.8-fold compared to in similarly treated wild-type cells

growth/size/body

decreased body size ( J:161240 )

decreased body weight ( J:161240 )

postnatal growth retardation ( J:161240 )

endocrine/exocrine glands

increased thymocyte apoptosis ( J:161240 )

• thymocytes exhibit increased radiosensitivity compared with similarly treated wild-type cells

decreased thymocyte number ( J:161240 )

increased mammary adenocarcinoma incidence ( J:161240 )

• in 1 mouse

abnormal seminiferous tubule epithelium morphology ( J:161240 )

♂ • disorganized

♂ • few spermatogonia are found within the seminiferous epithelium unlike in wild-type mice

seminiferous tubule degeneration ( J:161240 )

♂ • in severely affected mice without inflammation

small testis ( J:161240 )

♂

increased T cell derived lymphoma incidence ( J:161240 )

• in 3 mice

hematopoietic system

increased thymocyte apoptosis ( J:161240 )

• thymocytes exhibit increased radiosensitivity compared with similarly treated wild-type cells

abnormal class switch recombination ( J:161240 )

• class switch recombination is impaired compared to in wild-type mice

decreased pro-B cell number ( J:161240 )

decreased bone marrow cell number ( J:161240 )

decreased B cell number ( J:161240 )

decreased immature B cell number ( J:161240 )

decreased pre-B cell number ( J:161240 )

decreased T cell number ( J:161240 )

decreased thymocyte number ( J:161240 )

decreased double-negative T cell number ( J:161240 )

decreased double-positive T cell number ( J:161240 )

decreased CD4-positive, alpha-beta T cell number ( J:161240 )

decreased CD8-positive, alpha-beta T cell number ( J:161240 )

decreased splenocyte number ( J:161240 )

decreased IgG1 level ( J:161240 )

decreased IgG2b level ( J:161240 )

decreased IgG3 level ( J:161240 )

integument

increased mammary adenocarcinoma incidence ( J:161240 )

• in 1 mouse

increased skin tumor incidence ( J:161240 )

• in 1 mouse

homeostasis/metabolism

increased mortality induced by gamma-irradiation ( J:161240 )

• only 10% of mice treated with 8 Gy of gamma radiation are viable compared with 60% of similarly treated wild-type mice