Phenotypes Associated with This Genotype

Genotype
MGI:3799685

• Allelic Composition: Tg(ILK3mHEL)3Ccg/0; Tg(TcrHEL3A9)1Mmd/0
• Genetic Background: involves: C57BL/6JSfd * C57BL/10Sg * C57BR/cd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

insulitis ( J:78309 )

• 90% of islets are extensively infiltrated by T cells bearing transgenic TCRs, B cells and some macrophages

• however, there are no symptoms of diabetes

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• there is a reduced number of CD4-positive thymocytes

• CD4 T cell numbers in the lymph nodes are reduced by about half

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells expressing the transgenic TCR have a somewhat reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

hematopoietic system

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• there is a reduced number of CD4-positive thymocytes

• CD4 T cell numbers in the lymph nodes are reduced by about half

• the number of lymph node CD4 T cells bearing a transgenic TCR is reduced 2- to 4- fold compared to mice transgenic just for the TCR

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells expressing the transgenic TCR have a somewhat reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

endocrine/exocrine glands

insulitis ( J:78309 )

• 90% of islets are extensively infiltrated by T cells bearing transgenic TCRs, B cells and some macrophages

• however, there are no symptoms of diabetes

cellular

abnormal T cell proliferation ( J:78309 )

• CD4 T cells expressing the transgenic TCR have a somewhat reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene