Phenotypes Associated with This Genotype

Genotype
MGI:3799299

• Allelic Composition: Tg(LCKprBCL2)36Sjk/?
• Genetic Background: involves: C3H * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased T cell apoptosis ( J:11548 )

• 30% of thymocytes survive 6 days in culture compared to less than 1% for thymocytes from wild-type mice due to less apoptosis

• similar results are seen for splenic cells

• apoptosis induced by dexamethasone only causes a10-20% reduction in the number of thymocytes compared to 90 to 98% in control mice

• thymocytes are also resistant to apoptosis induced by low-dose radiation

• thymocytes are resistant to apoptosis triggered by CD3 ligation

abnormal thymus cell ratio ( J:11548 )

• ratio of CD4 to CD8 single positive T cells is 1.3 in transgenic mice compared to 3.9 in wild-type mice

abnormal T cell number ( J:28635 )

• at 12 months of age, there is a 3-4 fold increase in the absolute number of T cells in the spleen

• transgenic mice have 83 x 10⁶ T cells compared to 25 x 10⁶ T cells

abnormal T cell subpopulation ratio ( J:11548 )

• ratio of CD4 to CD8 T cells in the spleen is 1.4 compared to 2.6 in control mice

increased T cell number ( J:11548 )

• CD3^high/TCR^high cells make up 40% of thymocytes compared to 15% in non-transgenic controls

• there is a reciprocal decrease in CD3^lo cells in the thymus

• the number of T cells found in the spleen doubles

increased CD8-positive, alpha-beta T cell number ( J:11548 )

• the percentage of CD8 single positive T cells found in the thymus is almost double that in wild-type

• the percentage of CD8 T cells in the spleen is also doubled

neoplasm

increased T cell derived lymphoma incidence ( J:28635 )

• 18 of 68 mice develop lymphoma at a mean age of 18 months compared to 1 wild-type mouse out of 56 developing lymphoma in the control group

• 15 of the 18 lymphomas are large cell lymphoma with several showing immunoblastic features

• the remaining 3 are diffuse small lymphocytic lymphoma

• the lymphomas usually present as a rapidly expanding mass in the mesenteric lymph nodes though they can also occur in the mediastinal or cervical nodes

• extranodal involvement in other tissues occurs frequently

• splenomegaly and ascites fluid are sometimes observed

hematopoietic system

decreased T cell apoptosis ( J:11548 )

• 30% of thymocytes survive 6 days in culture compared to less than 1% for thymocytes from wild-type mice due to less apoptosis

• similar results are seen for splenic cells

• apoptosis induced by dexamethasone only causes a10-20% reduction in the number of thymocytes compared to 90 to 98% in control mice

• thymocytes are also resistant to apoptosis induced by low-dose radiation

• thymocytes are resistant to apoptosis triggered by CD3 ligation

abnormal thymus cell ratio ( J:11548 )

• ratio of CD4 to CD8 single positive T cells is 1.3 in transgenic mice compared to 3.9 in wild-type mice

abnormal T cell number ( J:28635 )

• at 12 months of age, there is a 3-4 fold increase in the absolute number of T cells in the spleen

• transgenic mice have 83 x 10⁶ T cells compared to 25 x 10⁶ T cells

abnormal T cell subpopulation ratio ( J:11548 )

• ratio of CD4 to CD8 T cells in the spleen is 1.4 compared to 2.6 in control mice

increased T cell number ( J:11548 )

• CD3^high/TCR^high cells make up 40% of thymocytes compared to 15% in non-transgenic controls

• there is a reciprocal decrease in CD3^lo cells in the thymus

• the number of T cells found in the spleen doubles

increased CD8-positive, alpha-beta T cell number ( J:11548 )

• the percentage of CD8 single positive T cells found in the thymus is almost double that in wild-type

• the percentage of CD8 T cells in the spleen is also doubled

cellular

decreased T cell apoptosis ( J:11548 )

• 30% of thymocytes survive 6 days in culture compared to less than 1% for thymocytes from wild-type mice due to less apoptosis

• similar results are seen for splenic cells

• apoptosis induced by dexamethasone only causes a10-20% reduction in the number of thymocytes compared to 90 to 98% in control mice

• thymocytes are also resistant to apoptosis induced by low-dose radiation

• thymocytes are resistant to apoptosis triggered by CD3 ligation

endocrine/exocrine glands

abnormal thymus cell ratio ( J:11548 )

• ratio of CD4 to CD8 single positive T cells is 1.3 in transgenic mice compared to 3.9 in wild-type mice

increased T cell derived lymphoma incidence ( J:28635 )

• 18 of 68 mice develop lymphoma at a mean age of 18 months compared to 1 wild-type mouse out of 56 developing lymphoma in the control group

• 15 of the 18 lymphomas are large cell lymphoma with several showing immunoblastic features

• the remaining 3 are diffuse small lymphocytic lymphoma

• the lymphomas usually present as a rapidly expanding mass in the mesenteric lymph nodes though they can also occur in the mediastinal or cervical nodes

• extranodal involvement in other tissues occurs frequently

• splenomegaly and ascites fluid are sometimes observed