Phenotypes Associated with This Genotype

Genotype
MGI:3795243

• Allelic Composition: Cnr1^tm1Zim/Cnr1^tm1Zim
• Genetic Background: involves: 129 * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

decreased survivor rate ( J:54995 )

• over time, mutants show an increase in spontaneous deaths, with more than 30% of mutants under 24 weeks of age dying

premature death ( J:54995 )

• over time, mutants show an increase in spontaneous deaths, with more than 30% of mutants under 24 weeks of age dying

• death is sudden with no signs of weight loss, dehydration, or abnormal posture

postnatal lethality, incomplete penetrance ( J:54995 )

• fewer than the expected numbers (23.3% vs. 29% expected) of homozygotes are seen at weaning, indicating some loss either during embryonic development or early postnatal life

reproductive system

early parturition ( J:144423 )

♀ • mice exhibit preterm labor prior to day 19.6 of pregnancy compared with wild-type mice

behavior/neurological

impaired behavioral response to addictive substance ( J:54995 )

• mutants show no hypothermia, no hypoactivity, and no catalepsy after administration of HU210, a potent synthetic cannabinoid agonist or delta9-tetrahydrocannabinol (THC) and no delta9-THC-induced analgesia in the hotplate test

• mutants exhibit head bobbing, assume a hunched position after injection, lick their abdomens, and have strong diarrhea after delta9-THC injection, unlike wild-type which show pronounced hypoactivity

decreased vertical activity ( J:55294 )

• rearing rate is reduced drastically

decreased locomotor activity ( J:54995 , J:55294 )

• mutants are hypoactive in the open-field test (J:54995)

• mutants exhibit a reduction in locomotor activity, in both ambulation counts and ambulation time (J:55294)

• however, motor coordination on the rotarod appears normal (J:55294)

hypoalgesia ( J:54995 )

• hypoalgesia in hotplate and formalin tests

• responses are normal in the tail-flick test

increased chemical nociceptive threshold ( J:54995 )

• mutants show a reduced number of pain responses in the early phase of the formalin test

increased thermal nociceptive threshold ( J:54995 )

• mutants show increased response latencies in the hotplate test

catalepsy ( J:54995 )

• mutants spend longer time motionless in the ring catalepsy test compared to wild-type indicating an increase in ring catalepsy

homeostasis/metabolism

increased circulating estradiol level ( J:144423 )

• on days 16 to 19 of pregnancy

increased circulating corticosterone level ( J:144423 )

• on days 14 to 16 of pregnancy

decreased circulating progesterone level ( J:144423 )

• on day 19 of pregnancy

increased follicle stimulating hormone level ( J:144423 )

• during late pregnancy

cellular

maternal effect ( J:144423 )

• newborns exhibit reduced birth weights compared to wild-type mice or newborns from homozygous females mated with wild-type mice

• however, treatment with a CRH-RI selective antagonist, antalarmin hydrochloride, on days 15 to 17 of pregnancy or progesterone on day 18 of pregnancy restores normal birth weights