Phenotypes Associated with This Genotype

Genotype
MGI:3794434

• Allelic Composition: Kitl^Sl-d/Kitl^Sl-d
• Genetic Background: C3.D2-Kitl^Sl-d

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

mortality/aging ( J:79293 )

N • homozygotes are viable with expected number of homozygotes observed at P1; 83% of mice survive to P18, similar to wild-type

hematopoietic system

macrocytic anemia ( J:79293 )

• severe at birth

low mean erythrocyte cell number ( J:79293 )

• significantly lower than wild-type at birth (32% of wild-type value)

decreased hematocrit ( J:79293 )

• significantly lower than wild-type at P24-25

increased mean corpuscular hemoglobin concentration ( J:79293 )

• significantly increased compared to wild-type at P24-25

increased mean corpuscular volume ( J:79293 )

• significantly increased compared to wild-type at P24-25

reproductive system

abnormal primordial germ cell morphology ( J:115437 )

• between E9.5 and 10.5, most PGCs are found with in the hindgut and these have abnormal morphology, while in wild-type embryos most PGCs are found in dorsal portions of mesentery

decreased primordial germ cell number ( J:115437 )

• moderate numbers of primordial germ cells (PGCs) are seen in genital ridges relative to wild-type and Kitl^Sl-gb homozygotes at E11.5

• at E9.5, PGCs are located primarily in the ventral axis of the hindgut while in wild-type PGCs are found primarily associated with the hindgut epithelium or in the dorsal axis of the hindgut; total PGC number in mutant embryos is 22% of wild-type

increased primordial germ cell apoptosis ( J:115437 )

• at E10.5, many PGCs in hindgut appear to be disintegrating; abnormal PGCs in hindgut tend to be nonmotile and apoptotic

abnormal primordial germ cell migration ( J:115437 )

• at E10.5, only 45% of total PGCs have migrated from hindgut, compared to 93% in wild-type

decreased primordial germ cell proliferation ( J:115437 )

• proliferation indices of migratory (in mesentery and genital ridges) and postmigratory PGCs (in genital ridges) at 10.5 and 11.5 are significantly reduced compared to wild-type values (54-66% of wild-type values)

cellular

abnormal primordial germ cell morphology ( J:115437 )

• between E9.5 and 10.5, most PGCs are found with in the hindgut and these have abnormal morphology, while in wild-type embryos most PGCs are found in dorsal portions of mesentery

decreased primordial germ cell number ( J:115437 )

• moderate numbers of primordial germ cells (PGCs) are seen in genital ridges relative to wild-type and Kitl^Sl-gb homozygotes at E11.5

• at E9.5, PGCs are located primarily in the ventral axis of the hindgut while in wild-type PGCs are found primarily associated with the hindgut epithelium or in the dorsal axis of the hindgut; total PGC number in mutant embryos is 22% of wild-type

increased primordial germ cell apoptosis ( J:115437 )

• at E10.5, many PGCs in hindgut appear to be disintegrating; abnormal PGCs in hindgut tend to be nonmotile and apoptotic

abnormal primordial germ cell migration ( J:115437 )

• at E10.5, only 45% of total PGCs have migrated from hindgut, compared to 93% in wild-type

decreased primordial germ cell proliferation ( J:115437 )

• proliferation indices of migratory (in mesentery and genital ridges) and postmigratory PGCs (in genital ridges) at 10.5 and 11.5 are significantly reduced compared to wild-type values (54-66% of wild-type values)