Phenotypes for Gli3 MGI:3784821 MGI Mouse

nervous system phenotype ( J:19818 )

N	• pituitary gland develops normally from floor of third ventricle • chiasmatic recess develops normally • mesencephalon does not appear enlarged relative to wild-type at 13.5 and 14.5 days of gestation

delayed neural tube closure ( J:19818 )

• anterior neuropore is still open to variable extent on day 11.5 of gestation in some embryos

• some embryos show normal closure while others display persistent open anterior neural folds spanning region of anterior neuropore to mesencephalon

incomplete rostral neuropore closure ( J:19818 )

• on day 13.5 of gestation, anterior neuropore remains open and brain ventricles openly communicate with the amniotic sac

abnormal rhombomere morphology ( J:137136 )

• loss of diagnostic morphology of isthmus

• anterior isthmus region with characteristics of the cerebellum

• thicker than normal Purkinje region and axons disorganized

• dorsal mesencephalon, isthmus, and r1 not distinct at E10.5 and E12.5

• isthmus flexure less prominent

abnormal choroid plexus morphology ( J:19818 )

• in embryos with closed anterior neuropore, forebrain does not show anlage of a choroid plexus in telencephalon

• small unpaired choroid plexus is found in the circumference of an opening in dorsal wall of diencephalon, not associated with origin of telencephalic anlagen from diencephalon

abnormal midbrain morphology ( J:137136 )

• tegmentum unaffected

• ventral nuclei containing dopaminergic neurons appear normal

enlarged cerebral aqueduct ( J:137136 )

• expanded mesencephalic ventricle at E10.5 and E12.5

abnormal inferior colliculus morphology ( J:137136 )

• loss of diagnostic morphology

• poorly formed

abnormal superior colliculus morphology ( J:137136 )

• loss of diagnostic morphology

enlarged tectum ( J:137136 )

• overgrown tectum

abnormal forebrain morphology ( J:19818 )

• on day 13.5, no choroid plexus is developing in forebrain

• hemispheres do not show any posterior elongation

abnormal telencephalon development ( J:19818 )

• in embryos with closed anterior neuropore, anlage of a choroid plexus is missing from telencephalon

• telencephalon is represented by a broad-based lateral bulging from the median portion of the forebrain and does not display elongation posteriorly

abnormal folding of telencephalic vesicles ( J:19818 )

• in embryos with closed anterior neuropore, forebrain does not show normal interhemispheric cleft

abnormal lateral ventricle morphology ( J:19818 )

• display poorly defined origin that opens wide into the median third ventricle

• smaller than wild-type on day 13.5 of gestation

abnormal cerebral hemisphere morphology ( J:19818 )

• at E15.5-16.5, cerebral hemispheres have very narrow rugged ventricles and do not show any lamination or cellular organization of the hemispheric walls

absent olfactory bulb ( J:19818 )

• bulb does not develop

abnormal hindbrain morphology ( J:137136 )

• thickness of ventricular zone increased

abnormal cerebellum morphology ( J:137136 )

• not clearly separated from the isthmus at E18.5

reduced cerebellar foliation ( J:137136 )

• poorly foliated

abnormal cerebellar Purkinje cell layer ( J:137136 )

• thicker than normal

• axons disorganized in lateral regions