Phenotypes Associated with This Genotype

Genotype
MGI:3775732

• Allelic Composition: Eomes^tm1.1Rob/Eomes⁺; Nodal^tm1Rob/Nodal⁺
• Genetic Background: involves: 129S/SvEv * 129S7/SvEvBrd * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

embryo

abnormal anterior visceral endoderm cell migration ( J:131055 )

• AVE is correctly induced but fails to migrate anteriorly

abnormal developmental patterning ( J:131055 )

• heart patterning abnormalities are observed by E9.5 due to loss of midline structures

abnormal germ layer development ( J:131055 )

• embryos lack visible germ layers

abnormal left-right axis patterning ( J:131055 )

• left-right patterning abnormalities are observed by E9.5 due to loss of midline structures

abnormal rostral-caudal patterning of the somites ( J:131055 )

• floor plate expansion results in increased spacing between somite rows at E9.5

rostral body truncation ( J:131055 )

• by E9.5, mutants lack head structures rostral to the otic placodes

rostral-caudal axis duplication ( J:131055 )

• in the most severely affected embryos, relatively complete duplications of the posterior body axis are observed, including extra somite rows

enlarged floor plate ( J:131055 )

• node abnormalities result in expansion of the floor plate of the neural tube

abnormal notochord morphology ( J:131055 )

• node duplications are accompanied by formation of an accessory notochord

abnormal primitive node morphology ( J:131055 )

• at E8.5, development of the node is severely disturbed in a subset of mutants

• complete node duplications are observed in some mutants

failure of primitive streak formation ( J:131055 )

• the most severely affected double heterozygotes show abnormalities around E7 and fail to form a primitive streak

increased somite number ( J:131055 )

• addition rows of somites are observed in the most severely affected embryos

disorganized embryonic tissue ( J:131055 )

• by late gastrulation stages, embryos are grossly disorganized

embryonic-extraembryonic boundary constriction ( J:131055 )

• the most severely affected embryos develop pronounced constrictions between the embryonic and extraembryonic regions of the conceptus

abnormal amniotic cavity morphology ( J:131055 )

• embryos frequently show tissue accumulation within the amniotic cavity

nervous system

enlarged floor plate ( J:131055 )

• node abnormalities result in expansion of the floor plate of the neural tube

cellular

abnormal anterior visceral endoderm cell migration ( J:131055 )

• AVE is correctly induced but fails to migrate anteriorly