Phenotypes Associated with This Genotype

Genotype
MGI:3765970

• Allelic Composition: Numb^tm1Ynj/Numb^tm1Ynj; Numbl^tm1Wmz/Numbl^tm1Wmz; Tg(Nes-cre/Esr1*)4Ynj/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

postnatal growth retardation ( J:127227 )

• by P14 with tamoxifen induction at P0, pups show growth retardation

nervous system

increased neuron apoptosis ( J:127227 )

• at P7, many striato-cortical junction cells are apoptotic

abnormal lateral ventricle morphology ( J:127227 )

• with tamoxifen induction at P0, by P14 induced animals show structural abnormalities along the lateral ventricle wall linings whereas uninduced animals do not

enlarged lateral ventricles ( J:127227 )

• induced animals show consistent lateral ventricle enlargement by P7 compared to uninduced controls where slight enlargement is occasionally observed; by P14, enlargement is severe

• by 6 weeks of age, enlargement is significantly reduced

abnormal olfactory bulb morphology ( J:127227 )

• at P14, induced mutants display smaller olfactory bulbs with fewer interneurons, compared to uninduced controls with comparable brain size

abnormal rostral migratory stream morphology ( J:127227 )

• at P14, the rostral migratory stream (RMS) of mutants contains fewer neuroblasts than controls

• in adult induced mutants (6 weeks old), there is recovery of neuroblast numbers in RMS into the olfactory bulb

abnormal postnatal subventricular zone morphology ( J:127227 )

• at P14, the rostral migratory stream (RMS) of mutants contains fewer neuroblasts than controls

• in adult induced mutants (6 weeks old), there is recovery of neuroblast numbers in RMS into the olfactory bulb

abnormal ependyma morphology ( J:127227 )

• at P7, induced animals show occasional gaps in the ependymal lining rather than a continuous lining; by P14, the gaps progress to ependymal detachment

• at P7, occasional defects in lateral wall integrity are seen compared to uninduced controls, but by P14, defects are severe with many areas having lost the ependyma and underlying subventricular zone (SVZ) niche

• at P7, induced mutants show a poorly formed ependyma with pseudocolumnar cells instead of a flat epithelial sheath; at P14, some ependymal cells detach and remaining cells form short, defective borders with separation between cells

• by 6 weeks of age, ventricular lining is composed of a multi-layered stratified epithelium instead of a single-cell layer seen in controls; epithelial barrier is composed of mixture of astrocytes and few ependymal cells, intermixed with scar tissue

cellular

increased neuron apoptosis ( J:127227 )

• at P7, many striato-cortical junction cells are apoptotic