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Phenotypes Associated with This Genotype
Genotype
MGI:3762122
Allelic
Composition		 Cops8tm1Nwe/Cops8tm1.1Nwe
Tg(Cd4-cre)1Cwi/?
Genetic
Background		 involves: 129S1/Sv * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cops8tm1.1Nwe mutation (0 available); any Cops8 mutation (20 available)
Cops8tm1Nwe mutation (1 available); any Cops8 mutation (20 available)
Tg(Cd4-cre)1Cwi mutation (10 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased T cell number ( J:126339 )
• mature T cell numbers in the spleen and lymph nodes are decreased compared to in Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• however, the proportion of T cells with naive, memory and regulatory phenotypes are normal
abnormal CD4-positive, alpha-beta T cell physiology ( J:126339 )
• CD4+ T cells are less responsive to IL-7 and exhibit a reduced survival compared to CD4+ T cells from IL-7-treated Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
abnormal T cell activation ( J:126339 )
• increase in cellular size following stimulation, an indication of activation, is substantially reduced in T cells compared to in T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
decreased T cell proliferation ( J:126339 )
• T cells stimulated with anti-CD3 and anti-CD28 or with phorbol 12-myristate 13-acetate (PMA) and ionomycin fail to proliferate as well as T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes even with the addition of IL-2
• cell cycling in T cells is reduced or absent compared to in T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• however, no increase in T cell apoptosis is observed
decreased interleukin-2 secretion ( J:126339 )
• T cells stimulated with anti-CD3 and anti-CD28 or with phorbol 12-myristate 13-acetate (PMA) and ionomycin produce less IL-2 than similarly treated wild-type cells

hematopoietic system
decreased T cell number ( J:126339 )
• mature T cell numbers in the spleen and lymph nodes are decreased compared to in Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• however, the proportion of T cells with naive, memory and regulatory phenotypes are normal
abnormal CD4-positive, alpha-beta T cell physiology ( J:126339 )
• CD4+ T cells are less responsive to IL-7 and exhibit a reduced survival compared to CD4+ T cells from IL-7-treated Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
abnormal T cell activation ( J:126339 )
• increase in cellular size following stimulation, an indication of activation, is substantially reduced in T cells compared to in T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
decreased T cell proliferation ( J:126339 )
• T cells stimulated with anti-CD3 and anti-CD28 or with phorbol 12-myristate 13-acetate (PMA) and ionomycin fail to proliferate as well as T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes even with the addition of IL-2
• cell cycling in T cells is reduced or absent compared to in T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• however, no increase in T cell apoptosis is observed

cellular
decreased T cell proliferation ( J:126339 )
• T cells stimulated with anti-CD3 and anti-CD28 or with phorbol 12-myristate 13-acetate (PMA) and ionomycin fail to proliferate as well as T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes even with the addition of IL-2
• cell cycling in T cells is reduced or absent compared to in T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• however, no increase in T cell apoptosis is observed

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
04/30/2024
MGI 6.23 		The Jackson Laboratory