Phenotypes Associated with This Genotype

Genotype
MGI:3762003

• Allelic Composition: Del(11Hoxb3-Hoxb4)1Karl/Del(11Hoxb3-Hoxb4)1Karl
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal definitive hematopoiesis ( J:83563 )

abnormal common myeloid progenitor cell morphology ( J:83563 )

• the absolute number of spleen colony forming units at day 12 was slightly reduced

• when cultured in a multicytokine mixture, Lin-Sca-1+c-Kit+ (LSK) bone marrow cells display a proliferative disadvantage relative to wild-type cells

decreased bone marrow cell number ( J:83563 )

• bone marrow cellularity is reduced by about 25% (34+/-5x10⁶ cells in two femurs compared to 45+/-6x10⁶ cells in two femurs in wild-type mice)

• however, cell proliferation is normal

decreased erythrocyte cell number ( J:83563 )

• 7.4+/-0.7x10¹² cells/L compared to 8.1+/-0.8x10¹² cells/L in wild-type mice

decreased hemoglobin content ( J:83563 )

• 139+/-10 g/L compared to 151+/-10 g/L in wild-type mice

abnormal hematopoietic stem cell morphology ( J:83563 )

• hematopoietic stem cells from mice treated with cyclophosphamide exhibit decreased proliferative capacity compared to cells from similarly treated wild-type mice

abnormal spleen morphology ( J:83563 )

decreased spleen weight ( J:83563 )

• spleen weight is reduced by 30% (70+/-7 mg compared to 103+/-14 mg in wild-type mice)

spleen hypoplasia ( J:83563 )

• spleen cellularity is reduced by 30% (127+/-24x10⁶ cells compared to 187+/-30x10⁶ cells in wild-type mice)

• however, cell proliferation is normal

liver/biliary system

abnormal liver morphology ( J:83563 )

• at E14.5, the number of Lin-ScaI+ AA4.1 cells is reduced (29+/-12x10³ cells compared to 49+/-22x10³ cells in wild-type mice)

growth/size/body

decreased body weight ( J:83563 )

• mice weight 8% less than wild-type mice at time of weaning

• however, this difference disappears by 12 weeks of age

skeleton

skeleton phenotype ( J:83563 )

N • unlike mice carrying Hoxb4^tm1.1Karl targeted mutation, formation of the thorax and other skeletal structures is normal

immune system

abnormal spleen morphology ( J:83563 )

decreased spleen weight ( J:83563 )

• spleen weight is reduced by 30% (70+/-7 mg compared to 103+/-14 mg in wild-type mice)

spleen hypoplasia ( J:83563 )

• spleen cellularity is reduced by 30% (127+/-24x10⁶ cells compared to 187+/-30x10⁶ cells in wild-type mice)

• however, cell proliferation is normal