Phenotypes Associated with This Genotype

Genotype
MGI:3760264

• Allelic Composition: Il2^tm1Hor/Il2^tm1Hor
• Genetic Background: B6.129P2-Il2^tm1Hor/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

digestive/alimentary system

abnormal salivary gland morphology ( J:125129 )

• severe acinar gland destruction

abnormal salivary gland duct morphology ( J:125129 )

• mild ductal changes in the salivary gland

large intestinal inflammation ( J:125129 )

• severe inflammation in the colon

• however, mice do show severe inflammation in the lung or the skin

decreased salivation ( J:125129 )

• mice exhibit an increase in the lag time and a reduction of saliva production when treated with Pilocarpine, indicating loss of salivary gland function

salivary gland inflammation ( J:125129 )

• severe inflammation in the salivary glands

• major population of infiltrating leukocytes are lymphocytes and to a much lower extent neutrophils

• salivary glands show presence of a large fraction of T cells and a ratio of CD4+/CD8+ of 1/2

• although more Gr-1+ cells are seen in the salivary glands, the absolute number of neutrophils is relatively small

immune system

large intestinal inflammation ( J:125129 )

• severe inflammation in the colon

• however, mice do show severe inflammation in the lung or the skin

salivary gland inflammation ( J:125129 )

• severe inflammation in the salivary glands

• major population of infiltrating leukocytes are lymphocytes and to a much lower extent neutrophils

• salivary glands show presence of a large fraction of T cells and a ratio of CD4+/CD8+ of 1/2

• although more Gr-1+ cells are seen in the salivary glands, the absolute number of neutrophils is relatively small

lacrimal gland inflammation ( J:125129 )

• severe inflammation in the lacrimal glands

increased CD4-positive, alpha-beta T cell number ( J:131774 )

• 3-fold

decreased regulatory T cell number ( J:113547 )

• there is a 2 to 5 fold reduction in the percentage of CD25-postive CD4 T cells in both the thymus and lymph nodes

decreased CD4-positive, CD25-positive, alpha-beta regulatory T cell number ( J:131774 )

• 4-fold

increased splenocyte number ( J:131774 )

abnormal T cell activation ( J:190168 )

• both CD4+ and CD8+ T cells fail to increase in size and granularity after activation with anti-CD3/28

abnormal T cell proliferation ( J:190168 )

• DNA replication fails to increase in activated CD4+ and CD8+ T cells after activation with anti-CD3/28

abnormal circulating cytokine level ( J:190168 )

decreased circulating interferon-gamma level ( J:190168 )

• after activation with anti-CD3/28

decreased circulating interleukin-13 level ( J:190168 )

• after activation with anti-CD3/28

decreased circulating interleukin-4 level ( J:190168 )

• after activation with anti-CD3/28

enlarged lymph nodes ( J:113547 )

• increased cellularity of lymph nodes compared to wild-type mice, but lower than what is observed in Il2rb^tm1Mak mice

increased susceptibility to autoimmune disorder ( J:113547 )

• generalized symptoms of autoimmunity noted at 4 weeks of age

• transfer of wild-type regulatory T cells did not prevent autoimmune disorder

abnormal response to transplant ( J:113547 )

• wild-type regulatory T cells fail to engraft when adoptively transferred into these mice

endocrine/exocrine glands

abnormal salivary gland morphology ( J:125129 )

• severe acinar gland destruction

abnormal salivary gland duct morphology ( J:125129 )

• mild ductal changes in the salivary gland

decreased salivation ( J:125129 )

• mice exhibit an increase in the lag time and a reduction of saliva production when treated with Pilocarpine, indicating loss of salivary gland function

salivary gland inflammation ( J:125129 )

• severe inflammation in the salivary glands

• major population of infiltrating leukocytes are lymphocytes and to a much lower extent neutrophils

• salivary glands show presence of a large fraction of T cells and a ratio of CD4+/CD8+ of 1/2

• although more Gr-1+ cells are seen in the salivary glands, the absolute number of neutrophils is relatively small

lacrimal gland inflammation ( J:125129 )

• severe inflammation in the lacrimal glands

cellular

abnormal T cell proliferation ( J:190168 )

• DNA replication fails to increase in activated CD4+ and CD8+ T cells after activation with anti-CD3/28

abnormal cellular respiration ( J:190168 )

• fail to switch from oxidative phosphorylation to aerobic glycolysis after 5 days of tamoxifen treatment and activation with anti-CD3/28

vision/eye

lacrimal gland inflammation ( J:125129 )

• severe inflammation in the lacrimal glands

homeostasis/metabolism

decreased salivation ( J:125129 )

• mice exhibit an increase in the lag time and a reduction of saliva production when treated with Pilocarpine, indicating loss of salivary gland function

abnormal circulating cytokine level ( J:190168 )

decreased circulating interferon-gamma level ( J:190168 )

• after activation with anti-CD3/28

decreased circulating interleukin-13 level ( J:190168 )

• after activation with anti-CD3/28

decreased circulating interleukin-4 level ( J:190168 )

• after activation with anti-CD3/28

hematopoietic system

increased CD4-positive, alpha-beta T cell number ( J:131774 )

• 3-fold

decreased regulatory T cell number ( J:113547 )

• there is a 2 to 5 fold reduction in the percentage of CD25-postive CD4 T cells in both the thymus and lymph nodes

decreased CD4-positive, CD25-positive, alpha-beta regulatory T cell number ( J:131774 )

• 4-fold

increased splenocyte number ( J:131774 )

abnormal T cell activation ( J:190168 )

• both CD4+ and CD8+ T cells fail to increase in size and granularity after activation with anti-CD3/28

abnormal T cell proliferation ( J:190168 )

• DNA replication fails to increase in activated CD4+ and CD8+ T cells after activation with anti-CD3/28

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Sjogren's syndrome		DOID:12894	OMIM:270150	J:125129