Analysis Tools
mortality/aging
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• >50% of transgenic mice die by ~100 days of age compared to ~7% of nontransgenic littermates
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behavior/neurological
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• upon transfer to new cage, many mice show neophobia
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• males older than 2 months display increased aggressiveness towards other males, with frequent and severe fighting
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• significantly more (~4%) mice older than ~2 months display long latency of >5 seconds when placed in a new environment compared to 5% of control FVB mice, whereas 4-6 week old mice show similar latency to control animals
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• after 2 months of age, mice display increased agitation and bouts of wild running
• in open field tests, mice 6-20 weeks old display significantly reduced ambulation, with number of corner crossings skewed toward lower values relative to controls which show a Gaussian distribution
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• upon transfer to new cage, many mice show postural freezing; significantly more mice 3-5 months of age display posture freezing compared to 2% of wild-type controls
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• after 2 months of age, >60% of mice show mild seizures of varying severity compared to very few in transgenic mice on the C57BL background
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• less susceptible than wild-type or line 6 transgenic mice to NMDA-induced seizures and mortality at all doses of NMDA treatment, displaying no symptoms and dying only when given doses of 150mg/kg
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• mice are more sensitive to kainic acid than wild-type, with all mice displaying stage 5 seizures at a dose of 40 mg/kg compared to only 30% of wild-type exhibiting stage 5 and 66% having stage 4 seizures
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• mice older than 6 months exhibit tonic-clonic seizures of 30-60 seconds, followed by whole body clonic seizures
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• many mice display seizures with facial movements, mild fore-limb clonus and mild whole body clonus lasting from 5 to 10 seconds
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• mice older than 6 months show severe tonic-clonic seizures lasting 30-60 seconds, with gasping and stretching all four limbs out with their tail upright, followed by severe whole body clonic seizures
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nervous system
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• after 2 months of age, >60% of mice show mild seizures of varying severity compared to very few in transgenic mice on the C57BL background
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• less susceptible than wild-type or line 6 transgenic mice to NMDA-induced seizures and mortality at all doses of NMDA treatment, displaying no symptoms and dying only when given doses of 150mg/kg
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• mice are more sensitive to kainic acid than wild-type, with all mice displaying stage 5 seizures at a dose of 40 mg/kg compared to only 30% of wild-type exhibiting stage 5 and 66% having stage 4 seizures
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• mice older than 6 months exhibit tonic-clonic seizures of 30-60 seconds, followed by whole body clonic seizures
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• many mice display seizures with facial movements, mild fore-limb clonus and mild whole body clonus lasting from 5 to 10 seconds
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• mice older than 6 months show severe tonic-clonic seizures lasting 30-60 seconds, with gasping and stretching all four limbs out with their tail upright, followed by severe whole body clonic seizures
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muscle
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• many mice display seizures with facial movements, mild fore-limb clonus and mild whole body clonus lasting from 5 to 10 seconds
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respiratory system
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• mice of all ages exhibit markedly increased respiratory rate in a new environment
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