mortality/aging
• number of offspring is less than the expected 25%; percentage of female offspring is 15-16%
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growth/size/body
• at 5 weeks of age, a 15-20% reduction in body weight is observed relative to wild-type mice
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hematopoietic system
• thymus wet weight is significantly less than in wild-type; weight is decreased 2.5-fold
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• decreased number of lymphoid cells; thymi are hypocellular
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• 87% reduction in number of more mature B220+ IgM+ B cells
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• number of lymphoid cells is decreased 7-fold; mutants have only ~50% as many bone marrow cells as wild-type mice at 5-6 weeks
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• red blood cell count is 82% of wild-type; 2-fold decrease in number of erythrocytes is found at 5-6 weeks
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• WBC count is only 30% of wild-type
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• 1.2-fold decrease
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• concentration of neutrophils is 60% of wild-type value
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• lymphocyte concentration is reduced to 37% of wild-type
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• 40% reduction in CD43+ B220+ immature B cells in bone marrow relative to wild-type
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• reduced to 40% of wild-type number
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• thymocyte number is 5% of wild-type
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• number of mature, double-negative thymocytes is reduced by >80% compared to wild-type
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• double positive thymocyte numbers are reduced in relative proportion from 40% in wild-type to 20% in mutants at 5-6 weeks
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• spleen wet weight is significantly less than in wild-type
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• decreased number of lymphoid cells
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immune system
• thymus wet weight is significantly less than in wild-type; weight is decreased 2.5-fold
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• decreased number of lymphoid cells; thymi are hypocellular
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• 87% reduction in number of more mature B220+ IgM+ B cells
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• WBC count is only 30% of wild-type
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• 1.2-fold decrease
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• concentration of neutrophils is 60% of wild-type value
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• lymphocyte concentration is reduced to 37% of wild-type
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• 40% reduction in CD43+ B220+ immature B cells in bone marrow relative to wild-type
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• reduced to 40% of wild-type number
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• thymocyte number is 5% of wild-type
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• number of mature, double-negative thymocytes is reduced by >80% compared to wild-type
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• double positive thymocyte numbers are reduced in relative proportion from 40% in wild-type to 20% in mutants at 5-6 weeks
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• spleen wet weight is significantly less than in wild-type
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• decreased number of lymphoid cells
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liver/biliary system
• liver wet weight is less than in wild-type
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renal/urinary system
• kidney wet weight is less than in wild-type
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cellular
• an increase in G1 arrest function is seen, with number of S-phase cells 72% of wild-type MEFs
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• exposure to 10 Gy of radiation results in 100% mortality within 7-9 days of treatment, compared to ~50% mortality in wild-type mice between 12 and 22 days after treatment
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• MEFs in culture show decreased rate of proliferation compared to wild-type or Mdm2-heterozygous cells
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endocrine/exocrine glands
• thymus wet weight is significantly less than in wild-type; weight is decreased 2.5-fold
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• decreased number of lymphoid cells; thymi are hypocellular
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• thymocyte number is 5% of wild-type
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