Phenotypes Associated with This Genotype

Genotype
MGI:3715012

• Allelic Composition: Itgb1^tm1Ref/Itgb1^tm1Ref; Tg(Ins2-cre)1Heed/?; Tg(RIP1-Tag)2Dh/?
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * C57BL/6J * CBA * DBA/2J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal cell morphology ( J:122649 )

• derived cells have fewer projections

decreased cell adhesion ( J:122649 )

• derived cell lines exhibit decreased binding to collagen IV

abnormal mitosis ( J:122649 )

• more derived tumor cells are arrested in G0/G1 than in Itgb1^tm1Ref Tg(RIP1-Tag)2Dh controls

abnormal cell death ( J:122649 )

• cultured cells die within a week after plating compared to Itgb1^tm1Ref Tg(RIP1-Tag)2Dh derived cells

early cellular replicative senescence ( J:122649 )

• more derived tumor cells are arrested in G0/G1 than in Itgb1^tm1Ref Tg(RIP1-Tag)2Dh controls

neoplasm

decreased metastatic potential ( J:122649 )

• when derived cell lines are injected into nu/nu mice, metastatic nodules from do not form in the lungs and or liver as they do in wild-type mice

abnormal tumor susceptibility ( J:122649 )

• 60% of mice have an increase in the number of disseminated tumor cell emboli within lymphatic vessels near tumors compared to 7.7% in Itgb1^tm1Ref Tg(RIP1-Tag)2Dh mice

• however, no metastasis is detected in mice with tumors

• tumor volume is reduced compared to that in Itgb1^tm1Ref Tg(RIP1-Tag)2Dh mice

• tumor cell proliferation is significantly reduced and apoptotic rate is reduced compared to Itgb1^tm1Ref Tg(RIP1-Tag)2Dh mice