Phenotypes Associated with This Genotype

Genotype
MGI:3714321

• Allelic Composition: Espl1^tm1.2Tno/Espl1^tm1.2Tno
• Genetic Background: involves: 129S4/SvJae * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality, complete penetrance ( J:107917 )

• although homozygous blastocysts are present at E3.5, no homozygous embryos are recovered at E8.5

embryo

abnormal inner cell mass proliferation ( J:107917 )

• trophoblasts do not spread in a E3.5 embryo after day 3 in culture

abnormal blastocyst morphology ( J:107917 )

• blastocyst are smaller than normal

• E3.5 embryos after day 1 in culture have 10% of the cells in a normal blastocyst and have enlarged nuclei by 2 times the normal diameter

• E3.5 embryos after day 3 in culture fail to either expand the inner cell mass and trophoblasts do not spread

cellular

abnormal cell nucleus size ( J:107917 )

• E3.5 embryos after day 1 in culture have enlarged nuclei by 2 times the normal diameter

abnormal inner cell mass proliferation ( J:107917 )

• trophoblasts do not spread in a E3.5 embryo after day 3 in culture