Analysis Tools
mortality/aging
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• mice die at approximately 3-4 weeks of age
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hematopoietic system
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• vigorous spontaneous proliferation of lymph node cells is seen in culture, with moderate proliferation of spleen cells in culture, compared to no spontaneous proliferation of cultured wild-type cells; ~80% of cells are CD4+CD8- single-positive cells and 20% are CD4-CD8+ single-positives after 6 days in culture
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• massive splenomegaly observed in 15 to 20-day old mice
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• mutant T cells are larger than wild-type T cells
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• increase is seen in percentage and expression levels of activation markers CD69, CD25, and CD44 on T cells in spleen and lymph nodes
• mutant T cells are larger than wild-type T cells
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• 10-fold decrease in percentage of double-positive CD4+CD8+ T cell in thymus is observed
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• relative increase in numbers of CD3+CD4+ and CD3+CD8+ single-positive T cells is seen in thymus
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• there is a 5-fold increase in CD4-CD8- double-negative cells in the thymus in 15 to 20-day old mice
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• increase in percentage and absolute number of CD3+ cells
• significant decrease in Mel-14+ (naive) T cells in spleen
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immune system
myocarditis
(
J:30393
)
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• destructive myocarditis is seen with massive interstitial infiltrates consisting of abundant CD3+ cells, CD4+ and CD8+ cells, numerous macrophages, and occasional B220+ B cells, as well as neutrophils in mice at ~15 days of age
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• vigorous spontaneous proliferation of lymph node cells is seen in culture, with moderate proliferation of spleen cells in culture, compared to no spontaneous proliferation of cultured wild-type cells; ~80% of cells are CD4+CD8- single-positive cells and 20% are CD4-CD8+ single-positives after 6 days in culture
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• some interstitial mononuclear infiltrates in absence of tissue destruction
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• severe pancreatitis is seen with intense mononuclear infiltrate with destruction of islets and glandular elements; infiltrates consist of abundant CD3+ cells, CD4+ and CD8+ cells, numerous macrophages, and occasional B220+ B cells at ~15 days of age
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• massive splenomegaly observed in 15 to 20-day old mice
|
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• mutant T cells are larger than wild-type T cells
|
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• increase is seen in percentage and expression levels of activation markers CD69, CD25, and CD44 on T cells in spleen and lymph nodes
• mutant T cells are larger than wild-type T cells
|
|
• 10-fold decrease in percentage of double-positive CD4+CD8+ T cell in thymus is observed
|
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• relative increase in numbers of CD3+CD4+ and CD3+CD8+ single-positive T cells is seen in thymus
|
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• there is a 5-fold increase in CD4-CD8- double-negative cells in the thymus in 15 to 20-day old mice
|
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• increase in percentage and absolute number of CD3+ cells
• significant decrease in Mel-14+ (naive) T cells in spleen
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• increase in percentage and absolute number of CD3+ cells at ~15 days of age
• significant decrease in Mel-14+ (naive) T cells in spleen
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• massive lymphadenopathy found, with up to 10-fold more lymph node cells in mutants
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• analysis of supernatants from anti-CD3-stimulated lymph node and spleen cells show increased amounts of interferon gamma (Ifng), interleukin-4 (Il-4), and granulocyte-macrophage colony-stimulating factor compared to cells from wild-type mice
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• synovitis observed in some mice
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• some interstitial mononuclear infiltrates in absence of tissue destruction are seen
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endocrine/exocrine glands
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• some interstitial mononuclear infiltrates in absence of tissue destruction
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• severe pancreatitis is seen with intense mononuclear infiltrate with destruction of islets and glandular elements; infiltrates consist of abundant CD3+ cells, CD4+ and CD8+ cells, numerous macrophages, and occasional B220+ B cells at ~15 days of age
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cardiovascular system
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• in some mice
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myocarditis
(
J:30393
)
|
• destructive myocarditis is seen with massive interstitial infiltrates consisting of abundant CD3+ cells, CD4+ and CD8+ cells, numerous macrophages, and occasional B220+ B cells, as well as neutrophils in mice at ~15 days of age
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digestive/alimentary system
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• some interstitial mononuclear infiltrates in absence of tissue destruction
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respiratory system
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• some interstitial mononuclear infiltrates in absence of tissue destruction are seen
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skeleton
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• synovitis observed in some mice
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growth/size/body
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• massive splenomegaly observed in 15 to 20-day old mice
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cellular
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• vigorous spontaneous proliferation of lymph node cells is seen in culture, with moderate proliferation of spleen cells in culture, compared to no spontaneous proliferation of cultured wild-type cells; ~80% of cells are CD4+CD8- single-positive cells and 20% are CD4-CD8+ single-positives after 6 days in culture
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