Analysis Tools
immune system
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• reactive oxygen species (ROS) production in unprimed, TNFalpha- or GM-CSF-primed bone marrow derived neutrophils (BMNs) is 69-84% reduced compared to controls; phorbyl myristate acetate (PMA) induced ROS production is substantially reduce compared to wild-type
• defective ROS generation in response to opsonized and unopsonized zymosan, S.aureus, or IgG latex beads is similar between primed and unprimed cells; difference in ROS response relative to wild-type is increased significantly upon opsonization of S aureus particles
• BMN neurophils adherent on fibrinogen are defective in ability to produce ROS upon stimulation by TNFalpha (or by mGM-CSF or fMLP)
• in vitro, ability of neutrophils to kill S.aureus is reduced by ~75% while killing of E.coli shows little defect
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• mice have significant defect in ability to clear a S. aureus infection in vivo; number of surviving bacterium 24 hours after peritoneal injection of live S.aureus is significantly greater than in wild-type controls
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hematopoietic system
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• reactive oxygen species (ROS) production in unprimed, TNFalpha- or GM-CSF-primed bone marrow derived neutrophils (BMNs) is 69-84% reduced compared to controls; phorbyl myristate acetate (PMA) induced ROS production is substantially reduce compared to wild-type
• defective ROS generation in response to opsonized and unopsonized zymosan, S.aureus, or IgG latex beads is similar between primed and unprimed cells; difference in ROS response relative to wild-type is increased significantly upon opsonization of S aureus particles
• BMN neurophils adherent on fibrinogen are defective in ability to produce ROS upon stimulation by TNFalpha (or by mGM-CSF or fMLP)
• in vitro, ability of neutrophils to kill S.aureus is reduced by ~75% while killing of E.coli shows little defect
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