Phenotypes Associated with This Genotype

Genotype
MGI:3706576

• Allelic Composition: Arpc3^{Tn(sb-lacZ,GFP)T5.117Jtak}/Arpc3^{Tn(sb-lacZ,GFP)T5.117Jtak}
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality before implantation, complete penetrance ( J:111402 )

• no live homozygous embryos are detected at 5.5 dpc (days post coitum); blastocysts can be isolated at 3.5 dpc

embryo

abnormal blastocyst morphology ( J:111402 )

• early stage blastocyts are indistinguishable from wild-type, but at later stages remain unexpanded at later stages of development

• when 3.5 dpc blastocysts are grown in culture, ~64% of blastocysts fail to hatch from their zona pellucida

• when mutant blastocysts are grown in culture after zona removal, blastocyst outgrowth is severely impaired even after 3 days in culture; cell number is comparable to wild-type blastocysts at 3 days in culture, indicating defect is due to abnormal spreading

abnormal inner cell mass morphology ( J:111402 )

• inner cell mass of homozygous blastocysts survive in culture, but growth is halted

• when grown on embryonic fibroblasts, ICM growth is delayed after 5 days compared to wild-type

cellular

abnormal cell morphology ( J:111402 )

• dynamics of actin accumulation are abnormal in mutant blastocysts at 3.5 dpc

• actin-rich adherent structures observed in abundance in normal cells during migration are drastically reduced at periphery of trophoblast cells in mutants

• cytoskeletal organization at cell periphery and actin net assembly are aberrant in mutant cells; actin filaments are <50 nm in diameter compared to 8 nm in wild-type cells