Phenotypes Associated with This Genotype

Genotype
MGI:3702265

• Allelic Composition: Tg(Tcra2D2,Tcrb2D2)1Kuch/0
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:83278 )

• 40% of transgenic mice succumb to EAE compared to no non-transgenic littermates

nervous system

CNS inflammation ( J:83278 )

• 7/15 mice without EAE show typical myelinating/demyelinating lesions of optic neuritis

• mice with EAE show typical myelinating/demyelinating lesions of optic neuritis

• 55 and 78% of transgenic mice immunized with 100 and 10 ug of MOG 35-55 without PT, respectively, develop optic nerve lesions

abnormal optic nerve morphology ( J:83278 )

• mice with or without EAE that display optic neuritis have myelinating/demyelinating lesions consisting of subpial and endoneurial mononuclear cell infiltrates with demyelination indicated by presence of foamy macrophages

axon degeneration ( J:83278 )

• mice with optic neuritis have varying degrees of axonal injury and loss

demyelination ( J:83278 )

• CNS tissues show myelin loss

vision/eye

blepharitis ( J:83278 )

• mice without EAE develop superficial inflammation around the eyelids; this is unilateral and not observed in wild-type littermates during up to 1 year observation

abnormal eye morphology ( J:83278 )

• superficial eye lesions in mice without EAE are often associated with progressive atrophy of the eye

• 67% of mutants show these eye lesions compared to no wild-type

eyelid edema ( J:83278 )

• mice without EAE show eyelid inflammation and eyelid swelling; this is unilateral and not observed in wild-type littermates during up to 1 year observation

abnormal optic nerve morphology ( J:83278 )

• mice with or without EAE that display optic neuritis have myelinating/demyelinating lesions consisting of subpial and endoneurial mononuclear cell infiltrates with demyelination indicated by presence of foamy macrophages

immune system

abnormal CD4-positive, alpha beta T cell morphology ( J:83278 )

• CD4/CD8 single positive ratio in thymus of transgenic mice is biased toward CD4+ compartment

• analysis shows a skewing toward CD4+ T cells in spleens as well

abnormal cytokine secretion ( J:83278 )

• spleen cells from naive mice produce high levels of IFN gamma in response to MOG 35-55

increased susceptibility to autoimmune disorder ( J:83278 )

• 4% (3/72) of mice develop spontaneous EAE, indicated initially by a limp tail, followed by hindlimb paralysis between 2.5 and 5 months of age

• 55 and 78% of mice immunized with 100 and 10 ug of MOG 35-55 without PT, respectively, develop optic nerve lesions

• mice with disease have typical myelinating/demyelinating lesions

increased susceptibility to experimental autoimmune encephalomyelitis ( J:83278 )

• transgenic mice immunized with MOG 35-55 + pertussis toxin (PT) develop more severe EAE than non-transgenic littermates, with earlier onset and greater clinical scores; 50% of mice develop associated optic neuritis also

• injection of PT alone induces clinical EAE in 39% and histological EAE in 56% of transgenics compared to no non-transgenic mice; 80% of mice develop associated optic neuritis also

CNS inflammation ( J:83278 )

• 7/15 mice without EAE show typical myelinating/demyelinating lesions of optic neuritis

• mice with EAE show typical myelinating/demyelinating lesions of optic neuritis

• 55 and 78% of transgenic mice immunized with 100 and 10 ug of MOG 35-55 without PT, respectively, develop optic nerve lesions

blepharitis ( J:83278 )

• mice without EAE develop superficial inflammation around the eyelids; this is unilateral and not observed in wild-type littermates during up to 1 year observation

hematopoietic system

abnormal CD4-positive, alpha beta T cell morphology ( J:83278 )

• CD4/CD8 single positive ratio in thymus of transgenic mice is biased toward CD4+ compartment

• analysis shows a skewing toward CD4+ T cells in spleens as well

cellular

increased cell proliferation ( J:83278 )

• spleen cells from naive mice show increased proliferative response to myelin oligodendrocyte protein peptide 35-55 (MOG 35-55) compared to wild-type mice

homeostasis/metabolism

edema ( J:83278 )

• EAE-affected mice showed edema in the brain and spinal cord

eyelid edema ( J:83278 )

• mice without EAE show eyelid inflammation and eyelid swelling; this is unilateral and not observed in wild-type littermates during up to 1 year observation

craniofacial

eyelid edema ( J:83278 )

• mice without EAE show eyelid inflammation and eyelid swelling; this is unilateral and not observed in wild-type littermates during up to 1 year observation

growth/size/body

eyelid edema ( J:83278 )

• mice without EAE show eyelid inflammation and eyelid swelling; this is unilateral and not observed in wild-type littermates during up to 1 year observation