Phenotypes Associated with This Genotype

Genotype
MGI:3698423

• Allelic Composition: Rr100^tm2.1Welm/Rr100^tm2.1Welm
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal T cell differentiation ( J:113136 )

• there is dramatic change in CD4 vs CD8 expression profile compared to wild-type

• a subpopulation of CD4 SP thymocytes show surface expression similar to double positive (DP) thymocytes (except lacking CD8 expression)

• intraepithelial lymphocytes (IEL) lose CD8a expression on TCR gamma/delta lineage cells whereas Cc8a^tm1.1Litt cells have normal CD8a levels

decreased double-positive T cell number ( J:113136 )

• percentage is decreased compared to controls (from 84% to 65%)

abnormal T cell subpopulation ratio ( J:113136 )

• in thymic lobes after 3 days in culture, ratio of CD4 single positive cells to double positive cells is much higher than in cultured heterozygous organs; percentage of immature CD8 SP thymocytes is seen

• "CD8-negative" SP cells emerge to a significant extent after 7 days of culture, coincident with development of double positive cells

increased CD4-positive, alpha-beta T cell number ( J:113136 )

• increased CD4 single positive (SP) cell number from 11% in controls to 29%

decreased CD8-positive, alpha-beta T cell number ( J:113136 )

• percentage (1.8%) and absolute numbers of CD8+ SP cells (1.9 x10⁶) in thymus of mutants are decreased compared to controls (4.5%, 2.8 x x10⁶ respectively)

• CD8+ T cell numbers in periphery (lymph nodes and spleen) are decreased vs controls

immune system

abnormal T cell differentiation ( J:113136 )

• there is dramatic change in CD4 vs CD8 expression profile compared to wild-type

• a subpopulation of CD4 SP thymocytes show surface expression similar to double positive (DP) thymocytes (except lacking CD8 expression)

• intraepithelial lymphocytes (IEL) lose CD8a expression on TCR gamma/delta lineage cells whereas Cc8a^tm1.1Litt cells have normal CD8a levels

decreased double-positive T cell number ( J:113136 )

• percentage is decreased compared to controls (from 84% to 65%)

abnormal T cell subpopulation ratio ( J:113136 )

• in thymic lobes after 3 days in culture, ratio of CD4 single positive cells to double positive cells is much higher than in cultured heterozygous organs; percentage of immature CD8 SP thymocytes is seen

• "CD8-negative" SP cells emerge to a significant extent after 7 days of culture, coincident with development of double positive cells

increased CD4-positive, alpha-beta T cell number ( J:113136 )

• increased CD4 single positive (SP) cell number from 11% in controls to 29%

decreased CD8-positive, alpha-beta T cell number ( J:113136 )

• percentage (1.8%) and absolute numbers of CD8+ SP cells (1.9 x10⁶) in thymus of mutants are decreased compared to controls (4.5%, 2.8 x x10⁶ respectively)

• CD8+ T cell numbers in periphery (lymph nodes and spleen) are decreased vs controls