Phenotypes Associated with This Genotype

Genotype
MGI:3696860

• Allelic Composition: Clec7a^tm1Yiw/Clec7a^tm1Yiw
• Genetic Background: C.129P2-Clec7a^tm1Yiw

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal macrophage physiology ( J:116670 )

• production of TNFa by thioglycollate-elicited macrophages treated with oxidized zymosan is abolished but is not altered with intact zymosan stimulation

• oxidized candidia does not elicit production of cytokines or ROS by mutant macrophages, but incubation with live C. albicans results in normal levels of production

• production of reactive oxygen species (ROS) by macrophages incubated with P. carinii is abolished compared to wild-type or Myd88 mutants

abnormal dendritic cell physiology ( J:116670 )

• production of Il12 and TNFa is abolished in bone marrow-derived dendritic cells (BMDC) after stimulation by Sparassis crispa glucan (SCG)

• SCG maturation of dendritic cells does not occur in mutants

abnormal cytokine secretion ( J:116670 )

• Il10 production is suppressed with LPS or zymosan stimulation of BMDCs compared to wild-type

increased susceptibility to fungal infection ( J:116670 )

• immunocompromised mutants infected with P. carinii have significantly more cysts in the lungs 24 hours after infection compared to wild-type

respiratory system

lung cyst ( J:116670 )

• immunocompromised mutants infected with P. carinii have significantly more cysts in the lungs 24 hours after infection compared to wild-type

hematopoietic system

abnormal macrophage physiology ( J:116670 )

• production of TNFa by thioglycollate-elicited macrophages treated with oxidized zymosan is abolished but is not altered with intact zymosan stimulation

• oxidized candidia does not elicit production of cytokines or ROS by mutant macrophages, but incubation with live C. albicans results in normal levels of production

• production of reactive oxygen species (ROS) by macrophages incubated with P. carinii is abolished compared to wild-type or Myd88 mutants

growth/size/body

lung cyst ( J:116670 )

• immunocompromised mutants infected with P. carinii have significantly more cysts in the lungs 24 hours after infection compared to wild-type