Phenotypes Associated with This Genotype

Genotype
MGI:3694122

• Allelic Composition: Cspg4^tm1Wbst/Cspg4^tm1Wbst
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

nervous system phenotype ( J:114437 )

N • mutants do not show significantly altered behavior or impaired neurogenesis in the hippocampus

abnormal dentate gyrus morphology ( J:114437 )

• more proliferating cells are found in the outer half of the granule cell layer (oGCL) than in control tissue where most cells are in the subgranular zone (SGZ) at 1 day after BrdU labeling; oGCL contains 5.9% of total BrdU-labeled cells compared to 1.9% in wild-type

• survival of dentate progenitor cells is reduced in mutants with only ~50% of BrdU-labeled cells surviving 4 weeks compared to wild-type

cellular

abnormal cell migration ( J:54319 )

• cultured aortic smooth muscle cells do not show enhanced migration in response to PDGF-AA whereas control cells show significant migration

decreased cell proliferation ( J:54319 )

• smooth muscle cells show significantly decreased proliferative responses to PDGF-AA compared to wild-type cells

homeostasis/metabolism

abnormal enzyme/coenzyme activity ( J:54319 )

• PDGF-AA activates ERK kinase activation only in wild-type, but not mutant aortic smooth muscle cells

• autophosphorylation of the PDGFalpha receptor is absent in mutant cells stimulated with PDGF-AA