Analysis Tools
cellular
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• the failure of zona pellucida formation by oocytes of mice lacking mouse ZP3 is rescued by expression of human ZP3, although these zonae appear thinner than those of wild-type oocytes or those expressing both mouse and human ZP3; the rescued zonae contain mouse ZP1 and ZP2
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reproductive system
| N |
• whereas female mice lacking ZP3 are infertile, those expressing human instead of mouse ZP3 produce normal offspring; in early generations their litter sizes were smaller than those of wild-type females or those expressing both mouse and human ZP3, but later studies have not borne out this observation
• whereas few or no eggs are recovered from gonadotropin-stimulated female mice lacking ZP3, similar numbers of eggs are retrieved from wild-type and human ZP3 rescue mice
• mouse sperm bind in vitro to cumulus-free ovulated oocytes in which mouse ZP3 has been replaced by human ZP3, but human sperm do not attach even transiently
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• the failure of zona pellucida formation by oocytes of mice lacking mouse ZP3 is rescued by expression of human ZP3, although these zonae appear thinner than those of wild-type oocytes or those expressing both mouse and human ZP3; the rescued zonae contain mouse ZP1 and ZP2
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embryo
| N |
• one- and two-celled embryos having the human protein in place of mouse ZP3, like wild-type embryos, do not continue to bind mouse sperm
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