Phenotypes Associated with This Genotype

Genotype
MGI:3652584

• Allelic Composition: Cd44^tm1Mak/Cd44^tm1Mak
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal leukocyte migration ( J:99032 )

• lower proportion of polarized cells in all directions or the direction of the N-formyl methionyl leucyl phenylalanine (fMLP) in the pool of mutant freshly isolated neutrophils compared with wild-type cells

• much more slower migration speed respond to fMLP stimulation and show random movement

• reduced numbers of neutrophil infiltrating the peritoneal cavity in response to fMLP and thioglycollate in vivo

abnormal definitive hematopoiesis ( J:72658 )

• altered tissue distribution of myeloid progenitors with increased levels of colony-forming unit-granulocyte-macrophage (CFU-GM) in bone marrow and reduced numbers of CFU-GM and CFU-macrophage (CFU-M) in spleen

• total number of colonies and the number of CFU-GM in the spleen after mobilization are reduced even further, whereas the number of all other hematopoietic progenitors from spleen and bone marrow are similar to controls

immune system

immune system phenotype ( J:72658 )

N • exhibit normal white blood cell counts, lymphocyte subsets in spleen and lymph nodes, thymocyte counts, Ig levels, and responses to VSV infection

abnormal leukocyte migration ( J:99032 )

• lower proportion of polarized cells in all directions or the direction of the N-formyl methionyl leucyl phenylalanine (fMLP) in the pool of mutant freshly isolated neutrophils compared with wild-type cells

• much more slower migration speed respond to fMLP stimulation and show random movement

• reduced numbers of neutrophil infiltrating the peritoneal cavity in response to fMLP and thioglycollate in vivo

increased susceptibility to parasitic infection ( J:72658 )

• develop exaggerated granuloma responses to Cryotosporidium parvum infection, with larger and greater numbers of granulomas

cellular

abnormal cell morphology ( J:105319 )

• cytoplasmic vacuolization in cultured primary fibroblasts, along with polygonal cell morphology

abnormal mitochondrial crista morphology ( J:105319 )

• mitochondria contain disrupted cristae in matrix

increased mitochondrial size ( J:105319 )

• enlarged mitochondria in colonocytes

abnormal cell physiology ( J:72658 )

• SV50-transformed mutant fibroblasts are more highly tumorigenic in nude mice than control fibroblasts

abnormal leukocyte migration ( J:99032 )

• lower proportion of polarized cells in all directions or the direction of the N-formyl methionyl leucyl phenylalanine (fMLP) in the pool of mutant freshly isolated neutrophils compared with wild-type cells

• much more slower migration speed respond to fMLP stimulation and show random movement

• reduced numbers of neutrophil infiltrating the peritoneal cavity in response to fMLP and thioglycollate in vivo

decreased fibroblast proliferation ( J:105319 )

• 50% decrease in the growth of viable cultured primary fibroblasts, compared with cells from wild-type mice

muscle

abnormal vascular smooth muscle physiology ( J:101783 )

• isolated smooth muscle cells exhibit impaired ability to compact collagen gels, to assemble fibrillar collagen from exogenous monomers, and to attach to gelatin

cardiovascular system

abnormal vascular smooth muscle physiology ( J:101783 )

• isolated smooth muscle cells exhibit impaired ability to compact collagen gels, to assemble fibrillar collagen from exogenous monomers, and to attach to gelatin