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Phenotypes Associated with This Genotype
Genotype
MGI:3639765
Allelic
Composition
Ascl1tm1And/Ascl1tm1And
Genetic
Background
involves: 129
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ascl1tm1And mutation (1 available); any Ascl1 mutation (27 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• at E11.5 and E12.5, numbers of TUNEL positive cells in the spinal cords of mutants are increased by factors of 3.2 and 9.7 respectively compared to controls, and apoptotic cells are seen primarily in the ventricular zone
• cultured precursor cells fail to produce neurons
• decreased proliferation in culture
• no CGRP-positive pulmonary neuroendocrine cells (including single and clustered PNECs) are detected in E13-E17 or neonatal lungs (J:64088)
• newborn mutants show complete lack of solitary airway neuroendocrine (NE) cells, as characterized by a panel of NE markers (J:113000)
• in contrast, enteric neurons and NE cells in the gut and pancreatic islets develop normally (J:113000)
• mutants show a defect in ventral telencephalic progenitors; progenitors are missing at E12.5
• in E10.5 embryos a dramatic increase in dI2 number is seen
• at E10.5 embryos show a 70% loss of dI3
• in E10.5 embryos a dramatic increase in dI4/6 interneuron number is seen
• at E10.5 embryos show a complete loss of dI5 neurons
• in E10.5 embryos a dramatic increase in dI4/6 interneuron number is seen
• V2 interneuron numbers are reduced to a similar extent as Ascl1tm1And

respiratory system
• newborn mutants show disruption of pulmonary neuroendocrine cell differentiation
• however, other bronchopulmonary cell types (e.g. Clara cells and type II alveolar cells) appear normal
• no clustered PNECs are detected in E13-E17 or neonatal lungs (J:64088)
• newborn mutants show complete lack of airway neuroepithelial bodies (normally clustering at airway branch points), as characterized by a panel of neuroendocrine markers (J:113000)

endocrine/exocrine glands
• no CGRP-positive pulmonary neuroendocrine cells (including single and clustered PNECs) are detected in E13-E17 or neonatal lungs (J:64088)
• newborn mutants show complete lack of solitary airway neuroendocrine (NE) cells, as characterized by a panel of NE markers (J:113000)
• in contrast, enteric neurons and NE cells in the gut and pancreatic islets develop normally (J:113000)

cellular
• at E11.5 and E12.5, numbers of TUNEL positive cells in the spinal cords of mutants are increased by factors of 3.2 and 9.7 respectively compared to controls, and apoptotic cells are seen primarily in the ventricular zone
• cultured precursor cells fail to produce neurons
• decreased proliferation in culture


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory