Phenotypes Associated with This Genotype

Genotype
MGI:3629734

• Allelic Composition: Plcg1^tm2Gcrp/Plcg1^tm2Gcrp
• Genetic Background: involves: 129/Sv * CD-1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:75316 )

• homozygotes die between E9.5 and E10.5

embryo

absent visceral yolk sac blood islands ( J:75316 )

• at E9.0, homozygotes show absence of blood islands in the yolk sac

abnormal embryonic hematopoiesis ( J:75316 )

• E9.0 mutant yolk sacs or embryo bodies exhibit significantly impaired primitive erythropoiesis, as shown by loss of expression of the Ter119 erythroid progenitor marker

hematopoietic system

abnormal embryonic hematopoiesis ( J:75316 )

• E9.0 mutant yolk sacs or embryo bodies exhibit significantly impaired primitive erythropoiesis, as shown by loss of expression of the Ter119 erythroid progenitor marker

abnormal proerythroblast morphology ( J:75316 )

• in vitro, CFU-E and BFU-E assays indicate that E8.0-E8.5 mutant yolk sacs or embryo bodies show a near absence of erythroid progenitors while formation of non-erythroid granulocyte/macrophage colonies remains unaffected

cardiovascular system

abnormal vascular endothelial cell development ( J:75316 )

• at E8.5, homozygotes display significantly impaired endothelial and hematopoietic development, as shown by complete loss of FLT1 expression in the yolk sac, and significantly reduced FLT1 expression in the embryo body

abnormal vasculogenesis ( J:75316 )

• at E8.5, homozygotes display significantly reduced vasculogenesis, as shown by loss of expression of the endothelial marker PECAM1 in vessels of the yolk sac, allantois, and embryo body