Mouse Genome Informatics
tg
    Tg(INS-MT2A,Tyr)1Pne/0
FVB-Tg(INS-MT2A,Tyr)1Pne
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
       
homeostasis/metabolism
• after a single dose of streptozocin, control animals become hyperglycemic (>400mg/dl) after 2 days, while transgenic animals do not acquire a similar degree of diabetes during the 6 days of observation
• islets from transgenic mice have 30-fold higher levels of metallothionein compared to control islets from FVB mice
• cultured transgenic islets are relatively protected from damage by streptozocin compared to control islets; control islets are visibly damaged by 0.25 mmol/l and disintegrate into single cells by 0.5 mmol/l streptozocin but transgenic islets don't exhibit a similar level of damage until exposure to a dose of 1 mmol/l
• transgenic islets have an increased threshold for DNA damage by streptozocin compared to control FVB islets

immune system
• transgenic islets transferred into streptozocin-induced diabetic BALB/c mice are able to maintain almost normal blood glucose levels for over 16 days in recipients, while control islets could maintain euglycemia for just over 8 days

endocrine/exocrine glands
• islets are resistant to morphological damage and cell death induced by incubation with SNAP, a nitric oxide donor; FVB control islets are damaged under the same conditions
• after transplantation of transgenic islet or control iset grafts into FVB recipients, after 6 days transgenic islets retain 60% of their original insulin content while control islets only retain less than 20% ot their initial insulin
• transgenic islets produce a lower level of reactive oxygen species after 7 hours of hypoxia than conrol islet cells

Mouse Models of Human Disease
OMIM IDRef(s)
Diabetes Mellitus, Insulin-Dependent; IDDM 222100 J:100251