Analysis Tools
cardiovascular system
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• mean arterial blood pressure as measured by telemetric sensors is singificantly higher in homozygous mutant mice than in wild-type littermates; administration of a nitric oxide synthase inhibitor results in greater elevation of the blood pressure of mutant than of wild-type mice
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• basal Ba2+ influx into single smooth muscle cells is greatly elevated in cells from thoracic aortae or cerebral aerteries of mutant mice versus from wild-type littermates, and increases further upon application of a diacylglycerol (DAG) analog; infection by adenovirus expressing siRNA against Trpc3 mRNA significantly diminishes the basal Ba2+ flux in mutant smooth muscle cells, but has no effect in wild-type cells
• whole-cell patch clamp recording of cerebral artery smooth muscle cells demonstrates higher basal current densities and a more depolarized membrane potential in mutant than in wild-type cells
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• although intravascular pressure causes a similar degree of vasoconstriction of cerebral arteries from wild-type and mutant littermates, the pressure at which myogenic dilatation reverses to myogenic constriction is significantly lower in mutant arteries
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• aortic rings and small mesenteric arteries from homozygous mutant mice are induced to greater contractility than are vessels from wild-type littermates by the alpha-1-adrenoreceptor agonist phenylephrine both in the absence and in the presence of the voltage-gated Ca2+ channel blocker CdCl2
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muscle
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• basal Ba2+ influx into single smooth muscle cells is greatly elevated in cells from thoracic aortae or cerebral aerteries of mutant mice versus from wild-type littermates, and increases further upon application of a diacylglycerol (DAG) analog; infection by adenovirus expressing siRNA against Trpc3 mRNA significantly diminishes the basal Ba2+ flux in mutant smooth muscle cells, but has no effect in wild-type cells
• whole-cell patch clamp recording of cerebral artery smooth muscle cells demonstrates higher basal current densities and a more depolarized membrane potential in mutant than in wild-type cells
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• although intravascular pressure causes a similar degree of vasoconstriction of cerebral arteries from wild-type and mutant littermates, the pressure at which myogenic dilatation reverses to myogenic constriction is significantly lower in mutant arteries
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• aortic rings and small mesenteric arteries from homozygous mutant mice are induced to greater contractility than are vessels from wild-type littermates by the alpha-1-adrenoreceptor agonist phenylephrine both in the absence and in the presence of the voltage-gated Ca2+ channel blocker CdCl2
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