Phenotypes Associated with This Genotype

Genotype
MGI:3620517

• Allelic Composition: Tg(CTSG-PML/RARA)135Ley/?
• Genetic Background: involves: C3H * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

enlarged spleen ( J:59080 )

• spleens of young (< 8mo), pre-leukemic transgenic mice are significantly enlarged

anemia ( J:59080 )

• peripheral blood hemoglobin concentrations are reduced in transgenic mice with leukemia

abnormal myeloblast morphology/development ( J:59080 )

• 68 +/- 7% of bone marrow cells from transgenic mice with leukemia are myelocytes or promyelocytes

thrombocytopenia ( J:59080 )

• platelet counts are diminished in transgenic mice with leukemia

increased leukocyte cell number ( J:59080 )

• transgenic mice with leukemia have elevated numbers of peripheral blood WBCs, a high proportion of them early myeloid-lineage cells

abnormal spleen red pulp morphology ( J:59080 )

• young (< 8 mo), pre-leukemic transgenic mice have elevated numbers of early myeloid cells in the red pulp of the spleen

immune system

immune system phenotype ( J:59080 )

N • Gr-1⁺ cells from transgenic mice with leukemia exhibit surface expression of MHC class I markers at levels similar to that of wild-type Gr-1⁺ spleen cells

enlarged spleen ( J:59080 )

• spleens of young (< 8mo), pre-leukemic transgenic mice are significantly enlarged

increased leukocyte cell number ( J:59080 )

• transgenic mice with leukemia have elevated numbers of peripheral blood WBCs, a high proportion of them early myeloid-lineage cells

abnormal spleen red pulp morphology ( J:59080 )

• young (< 8 mo), pre-leukemic transgenic mice have elevated numbers of early myeloid cells in the red pulp of the spleen

neoplasm

increased acute promyelocytic leukemia incidence ( J:59080 )

• 1 of 31 (3.2%) and 4 of 27 (15%) transgenic mice developed acute premyelocytic leukemia (APML) by 12 and 24 months of age, respectively

• the average latency to APML onset is 7.5 +/- 0.8 months

• the proportion of early myeloid-lineage cells is elevated in peripheral blood of transgenic mice with leukemia

• an abnormal population of Gr-1⁺ CD34⁺ cells is present in bone marrow and blood of transgenic mice with leukemia and accounts for a large proportion (34% and 45% in 2 animals) of leukemic spleen cells; histologic examination shows this population is enriched for immature myeloid cells

• spleen cells from transgenic mice with leukemia transmit APML resulting in death of 100% of unirradiated, syngeneic (C3H xC57BL/6)F1 mice

• culture of spleen cells from leukemic mice in the presence of all trans retinoic acid (ATRA) significantly increases the proportion of histologically identified late myeloid cells relative to those in untreated cultures

growth/size/body

enlarged spleen ( J:59080 )

• spleens of young (< 8mo), pre-leukemic transgenic mice are significantly enlarged