Mouse Genome Informatics
hm
    Neil1tm1Rsld/Neil1tm1Rsld
involves: 129S1/Sv * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
       

Neil1tm1Rsld/Neil1tm1Rsld and wild-type mouse comparison

growth/size
• obese mice contain massive deposition of fat within the subcutis, retroperitoneum, mediastinum, and abdomen, with nearly complete encasement of most internal organs
• males, and to a lesser extent females, develop obesity at around 6-10 months of age
• although sublines at Oregon Health & Science University and University of Texas Medical branch exhibit severe obesity, one breeding pair of homozygotes raised at New York University appears normal, even at 1 year of age

adipose tissue
• obese mice contain massive deposition of fat within the subcutis, retroperitoneum, mediastinum, and abdomen, with nearly complete encasement of most internal organs
• sporadically observe reduced subcutaneous fat only in the colony at Oregon Health & Science University

homeostasis/metabolism
• seen in 1 year old obese males but not in females
• by 6 months of age, all males exhibit hyperleptinemia
• females at 1 year of age, display approximately 2.5-fold increase in leptin levels
• triglyceride levels are 1.4- and 1.3-fold elevated at 3 months and 1.7- and 1.4-fold elevated at 7 months of age in males and females, respectively

liver/biliary system
• males display significantly increased levels of mitochondrial DNA damage and deletions in liver tissue
• males exhibit marked hepatic steatosis with more than 50% of cellular contents being a mixture of micro- and macrovesicular globules, however do not observe inflammation or fibrosis
• steatosis is most prominent in the centrilobular regions
• females exhibit a much less severe steatosis than males
• pale yellow-colored liver

renal/urinary system
• males, and to a lesser extent females, display cytoplasmic vacuolization of tubule cells causing distension in many epithelial cells of the proximal convoluted tubules

immune system
• sporadically seen only in the colony at Oregon Health & Science University

reproductive system
• sporadically seen only in the colony at Oregon Health & Science University

skeleton
• sporadically seen only in the colony at Oregon Health & Science University

tumorigenesis
• sporadically observe cancers only in the colony at Oregon Health & Science University

integument
• sporadically observe skin ulcerations only in the colony at Oregon Health & Science University