Analysis Tools
cellular
|
• in heterozygous MEFs after 5 passages about 21% of metaphases are aneuploid compared to about 9% in wild-type cells and a wider range of nonmodal chromosome numbers is seen
(J:81546)
• at 5 months of age, 9% of splenocytes are aneuploid
(J:81546)
• the number of aneuploid splenocytes increases from 9% at 5 months to 29% at 27 months compared to only 3% in wild-type mice at 35 months
(J:105717)
|
|
• after nocodazole treatment MEFs leave mitosis prematurely without undergoing cell division
(J:81546)
• overexpression of Rae1 can rescue the mitotic checkpoint defect
(J:81546)
• after nocodazole treatment the time for 50% of cells to exit prometaphase arrest is reduced to 3 hours compared to 7.2 hours for wild-type MEFs
(J:105717)
|
|
• lagging chromosomes are seen in 5% of anaphase figures compared to 2% of wild-type figures
(J:81546)
• in splenocytes prematurely separated sister chromatids increase from 0 at 5 months to being present in 10-11% of metaphases at 24-27 months compared to 0 at 27 months and only 4% at 35 months in wild-type
(J:105717)
• however, no chromosome breaks or fusions are seen at 24 months of age
(J:105717)
|
neoplasm
|
• a single application of 50 ul of 0.5% DMBA at P5 results in an increased incidence of lung tumors and more tumors per animal in heterozygotes compared to wild-type mice
• however, no difference in spontaneous tumor incidence is seen compared to wild-type mice
|
homeostasis/metabolism
|
• a single application of 50 ul of 0.5% DMBA at P5 results in an increased incidence of lung tumors and more tumors per animal in heterozygotes compared to wild-type mice
• however, no difference in spontaneous tumor incidence is seen compared to wild-type mice
|
