Mouse Genome Informatics
hm
    Nos1tm1Plh/Nos1tm1Plh
involves: 129S4/SvJae
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
       
mortality/aging
• about 80% survival rate at 10 months of age
• about 20% die before 10 months of age

homeostasis/metabolism
• following carotid artery ligation the extent of neointimal formation is increased and luminal narrowing is worsened compared to wild-type controls
• myocardial infarct size following 30 min of global ischemia is increased compared to controls
• the size of striatal lesions induced by malonate are significantly reduced compared to similarly treated controls
• insulin resistance in peripheral tissues
• 24 h after aspiration bulbectomy the number of apoptotic cells in the piriform cortex is reduced compared to similarly treated wild-type mice
• following occlusion of the middle cerebral artery infarct size is decreased

nervous system
N
• despite the ability of NOS inhibitors to decrease long term potentiation, no significant decrease in long term potentiation is detected at 1 h after tetanus (J:37956)
• unlike cerebral blood flow, the intensity of neural activation induced by perioral stimulation is not significantly different from controls (J:84255)
• 24 h after aspiration bulbectomy the number of apoptotic cells in the piriform cortex is reduced compared to similarly treated wild-type mice
• the size of striatal lesions induced by malonate are significantly reduced compared to similarly treated controls
• following occlusion of the middle cerebral artery infarct size is decreased
• following a theta burst protocol at a weak intensity enhancement of EPSPs during the first 3 minutes after stimulation is significantly small compared to controls
• however, no significant differences are detected when a stronger intensity stimulation is used
• stimulation of parallel fibers with a 50 msec postsynaptic depolarizations fails to induce long term depression in Purkinje cells
• addition of uncaged NO or photoreleased cGMP to the stimulation protocol also fails to induce long term depression in Purkinje cells, unlike in wild-type mice
• glutamate release after NMDA stimulation is significantly attenuated in the cerebral cortex, partially reduced in the striatum but unaffected in the hippocampus

behavior/neurological
N
• unlike in earlier reports males show no signs of increased aggression (J:42548)
• no defects in circadian rhythms are detected in a wheel running assay either under entrained or free running conditions (J:56005)
• mice show no improvement in their latency to fall off a pole or plank in the dark versus the light phase, unlike in wild-type controls resulting in deficits in balance compared to wild-type mice during the dark phase

digestive/alimentary system
• lower esophageal sphincters fail to display electrical field stimulation (60 V, 5 Hz) induced relaxation or rebound contraction (J:56948)
• the mean resting lower esophageal sphincter pressure is significantly higher (J:70182)
• swallowing induced lower esophageal sphincter relaxation is significantly attenuated (J:70182)
• mice show a range of responses to pharyngeal stimulation including no relaxation, partial relaxation , and sporadic near complete relaxation of the lower esophageal sphincter (J:70182)
• efferent vagal stimulation induced lower esophageal sphincter relaxation is significantly attenuated (J:70182)

immune system
• increase in baseline rolling
• treatment with an anti-P-selectin antibody significantly reduces the rolling response in mutant but not in wild-type mice
• treatment with a low dose of thrombin significantly increases rolling in mutant but not in wild-type mice
• dramatic increase in baseline leukocyte adherence
• treatment with an anti-P-selectin antibody significantly reduces the adhesion response in mutant but not in wild-type mice
• following thioglycollate injection neutrophil accumulation in the peritoneum is significantly enhanced compared to wild-type mice

muscle
• the resting membrane potential of jejunal smooth muscle cells is depolarized about 5 mV compared to wild type cells
• hyperpolarization and induction of an inhibitory junction potential in response to electrical field stimulation under nonadrenergic noncholinergic conditions in jejunal muscle strips are markedly reduced
• lower esophageal sphincters fail to display electrical field stimulation (60 V, 5 Hz) induced relaxation or rebound contraction (J:56948)
• the mean resting lower esophageal sphincter pressure is significantly higher (J:70182)
• swallowing induced lower esophageal sphincter relaxation is significantly attenuated (J:70182)
• mice show a range of responses to pharyngeal stimulation including no relaxation, partial relaxation , and sporadic near complete relaxation of the lower esophageal sphincter (J:70182)
• efferent vagal stimulation induced lower esophageal sphincter relaxation is significantly attenuated (J:70182)
• lower esophageal sphincters fail to display electrical field stimulation (60 V, 5 Hz) induced relaxation or rebound contraction (J:56948)
• the decrease in muscle contractions in jejunal muscle strips in response to electrical field stimulation is markedly reduced (J:89581)

cardiovascular system
N
• unlike in mice null for Nos3 no significant abnormalities in hypoxic pulmonary vasoconstriction, vasodilation in response to bradykinin, or right ventricle systolic pressure are detected (J:57624)
• the increase in cerebral blood flow induced by glutamate, perioral stimulation, or harmaline is significantly attenuated
• in isolated perfused hearts
• however, coronary flow per beat is not significantly different from controls
• following carotid artery ligation the extent of neointimal formation is increased and luminal narrowing is worsened compared to wild-type controls
• myocardial infarct size following 30 min of global ischemia is increased compared to controls

vision/eye
N
• no defects are detected in visual acuity (J:56005)

reproductive system
N
• unlike in Nos3 null mice, the increase in maximal penile intracavernous pressure induced by papaverine is not significantly different from wild-type controls (J:89704)

growth/size

cellular
• the size of striatal lesions induced by malonate are significantly reduced compared to similarly treated controls
• increase in baseline rolling
• treatment with an anti-P-selectin antibody significantly reduces the rolling response in mutant but not in wild-type mice
• treatment with a low dose of thrombin significantly increases rolling in mutant but not in wild-type mice
• dramatic increase in baseline leukocyte adherence
• treatment with an anti-P-selectin antibody significantly reduces the adhesion response in mutant but not in wild-type mice

hematopoietic system
• increase in baseline rolling
• treatment with an anti-P-selectin antibody significantly reduces the rolling response in mutant but not in wild-type mice
• treatment with a low dose of thrombin significantly increases rolling in mutant but not in wild-type mice
• dramatic increase in baseline leukocyte adherence
• treatment with an anti-P-selectin antibody significantly reduces the adhesion response in mutant but not in wild-type mice
• following thioglycollate injection neutrophil accumulation in the peritoneum is significantly enhanced compared to wild-type mice

Mouse Models of Human Disease
OMIM IDRef(s)
Achalasia, Familial Esophageal 200400 J:70182