Phenotypes Associated with This Genotype

Genotype
MGI:3606309

• Allelic Composition: Cebpd^tm1Pfj/Cebpd^tm1Pfj
• Genetic Background: involves: 129S1/Sv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

mortality/aging ( J:49799 )

N • homozygotes are viable, fertile and developmentally normal; no significant differences in body weight, core body temperature, or hematology are observed

behavior/neurological

abnormal contextual conditioning behavior ( J:49799 )

• at 3 months, homoygotes exhibit normal brain neurochemistry with respect to total monoamine content, as well as normal spatial learning (Morris water maze task), locomotor activity (open-field), motor function (rotarod), and anxiety-like behavior (light/dark exploration test)

• however, homozygotes exhibit a selective increase in Pavlovian conditioned fear, with markedly increased freezing levels after long (i.e 24-hr) retention intervals; no significant differences are noted during the 30-min context test

• notably, homozygotes do not exhibit enhanced auditory-cue-conditioned fear, and serotonin and norepinephrine levels remain normal in mutant cortex

homeostasis/metabolism

abnormal cytokine level ( J:192313 )

• TNF-alpha, IL-6, and MIP-2 levels are decreased by 85%, 60% and 45%, respectively, in mutants compared to wild-type mice following intratracheal instillation of LPS

decreased susceptibility to injury ( J:192313 )

• mutants exhibit reduced lung injury following intratracheal instillation of lipopolysaccharide (LPS), showing decreased neutrophil accumulation, negligible intra-alveolar hemorrhage, diminished albumin levels in bronchial alveolar lavage (BAL) fluids, decrease in MPO activity, fewer neutrophils but increased macrophages in BAL fluids, and decreased TNF-alpha, IL-6 and MIP-2 levels compared to similarly treated wild-type mice

immune system

abnormal cytokine level ( J:192313 )

• TNF-alpha, IL-6, and MIP-2 levels are decreased by 85%, 60% and 45%, respectively, in mutants compared to wild-type mice following intratracheal instillation of LPS

decreased inflammatory response ( J:192313 )

• mutants exhibit reduced inflammatory cell influx into the lungs following intratracheal instillation of LPS to induce lung damage compared to wild-type mice, showing attenuation of neutrophil accumulation

respiratory system

abnormal respiratory system physiology ( J:192313 )

• mutants exhibit reduced lung injury following intratracheal instillation of LPS, showing decreased neutrophil accumulation, negligible intra-alveolar hemorrhage, diminished albumin levels in BAL fluids, decrease in MPO activity, fewer neutrophils but increased macrophages in BAL fluids, and decreased TNF-alpha, IL-6 and MIP-2 levels compared to similarly treated wild-type mice