Phenotypes for Ptgs2 MGI:3603952 MGI Mouse

premature death ( J:104002 )

• about 20% of mice die between 7 and 23 weeks of age

reproductive system phenotype ( J:69034 )

N	• in vitro, oocyte-cumulus cell complexes retrieved from eCG-primed ovaries of adult homozygotes display normal follicular growth and oocyte maturation • in vitro-matured oocytes obtained from adult female homozygotes show no significant differences in fertilization or preimplantation development

abnormal primary polar body morphology ( J:43609 )

• extrusion of the first polar body is rarely noted

abnormal decidualization ( J:43609 )

• pseudopregnant female homozygotes fail to exhibit an increase in uterine weight in response to intraluminal infusion of oil (a deciduogenic stimulus)

• intraluminal infusion of PGI2 (but not PGE2) or cholera toxin as the deciduogenic stimulus partially restores decidualization in ovaroectomized steroid hormonally prepared mutant mice

decreased superovulation rate ( J:43609 , J:69034 )

• females homozygotes exhibit significantly reduced superovulation, despite normal ovarian response to gonadotropins (J:43609)

• any recovered eggs appear developmentally abnormal, with virtually no extrusion of the first polar body (J:43609)

• adult (2- to 8-month-old) female homozygotes exhibit a poor ovulation rate upon induction with eCG and hCG (J:69034)

• immature (3-week-old) female homozygotes exhibit a superior ovulation rate relative to adult homozygotes, suggesting that the ovulatory process becomes defective with aging (J:69034)

failure of embryo implantation ( J:43609 )

• in blastocyst transfer experiments, wild-type blastocysts fail to exhibit the initial attachment reaction and do not implant into uteri of pseudopregnant female homozygous mutant mice

• failure of implantation and subsequent decidualization occur despite normal uterine responsiveness to steroid hormones

reduced female fertility ( J:109548 , J:118171 )

• number of term pregnancies reduced (J:109548)

• only 20% of homozygous females can sustain term pregnancy (J:118171)

decreased litter size ( J:118171 )

• homozygous females produce small litters compared to wild-type

impaired fertilization ( J:43609 )

• female homozygotes display complete failure of fertilization, despite the presence of numerous sperm at the fertilization site

abnormal decidualization ( J:43609 )

• pseudopregnant female homozygotes fail to exhibit an increase in uterine weight in response to intraluminal infusion of oil (a deciduogenic stimulus)

• intraluminal infusion of PGI2 (but not PGE2) or cholera toxin as the deciduogenic stimulus partially restores decidualization in ovaroectomized steroid hormonally prepared mutant mice

renal/urinary system phenotype ( J:104002 )

N	• adult mice exhibit normal urinalysis and 24-hr urine output under non-stressed conditions • no significant differences in urine osmolarity or in daily urinary excretion of sodium, potassium and chloride are observed

kidney cyst ( J:104002 )

• early cystic changes affecting different tubule sections and glomeruli at P10, with slightly variable pathologic progression

• severe cyst formation by P28

kidney cortex cyst ( J:104002 )

• massive tubular cysts in severely affected kidneys at P14

renal glomerulus cyst ( J:104002 )

• by P14, all mice exhibit cystic subcapsular glomeruli

abnormal kidney cortex morphology ( J:104002 )

• by P14, all mice exhibit outer cortical dysplasia

• inner cortical nephron hypertrophy by P42

abnormal renal glomerulus morphology ( J:104002 , J:109548 , J:118171 )

• small, crowded glomeruli in subcapsular region at P10 (J:104002)

• outer cortical glomerular hypoplasia at P42 (J:104002)

• hypoplastic (J:109548)

• average glomerular diameter is reduced to 29.4 um from 39.2 um in wild-type (J:118171)

cortical renal glomerulopathies ( J:118171 )

• at >6 weeks of age, renal cortex is abnormal with small immature glomeruli and deteriorating tubules; these changes are not seen in 2-month old COX-1 knockin mice

glomerulosclerosis ( J:104002 , J:109548 )

• focally variable glomerular sclerosis by P42 (J:104002)

• however, no inflammatory infiltrate or vascular pathology is observed at any age (J:104002)

• moderate (J:109548)

renal glomerulus fibrosis ( J:104002 )

• peri-glomerular fibrosis by P42

renal glomerulus hypertrophy ( J:104002 )

• hypertrophy of juxtamedullary glomeruli at P28

• inner cortical glomerular hypertrophy by P42

abnormal kidney development ( J:104002 )

• mice exhibit progressive cystic dysplasia during the later stages of kidney development

• however, prenatal and early postnatal kidney development appears normal

renal interstitial fibrosis ( J:104002 )

• diffuse interstitial fibrosis by P42

small kidney ( J:109548 )

kidney cortex atrophy ( J:109548 )

decreased kidney weight ( J:104002 )

• starting at P10, total kidney mass is significantly reduced relative to that in wild-type controls

• kidney-specific growth suppression persists to P42 with no significant change

renal tubule atrophy ( J:104002 )

• by P14, all mice exhibit loss of proximal tubular mass

abnormal proximal convoluted tubule morphology ( J:104002 )

• variable loss of normal proximal tubule mantle at P10

proximal convoluted tubule brush border loss ( J:104002 )

• by P14, all mice exhibit loss of brush border definition

dilated renal tubule ( J:104002 )

• variable tubular dilation at P10

• severe diffuse tubular dilation by P42

renal tubule hypertrophy ( J:104002 )

• hypertrophy of juxtamedullary tubules by P28

kidney degeneration ( J:104002 )

• at 8 weeks of age, some mice exhibit more severe cystic degeneration than others

pale kidney ( J:109548 )

decreased renal glomerular filtration rate ( J:104002 )

• adult mice exhibit a ~50% reduction in GFR relative to wild-type controls, as measured by inulin clearance

kidney failure ( J:104002 )

• mice exhibit progressive renal insufficiency

cardiovascular system phenotype ( J:104002 )

N	• normal systolic blood pressure in awake or anesthetized mice relative to wild-type controls

patent ductus arteriosus ( J:109548 )

• fails to close in 40% of mice

increased heart weight ( J:104002 )

• at birth, mice exhibit a significantly higher heart weight:body weight ratio relative to control mice

• however, a normal ratio is observed during postnatal growth and early adulthood

decreased vascular permeability ( J:118171 )

• dye extravasation in the ear vasculature is decreased by 50-60% with bradykinin challenge compared to wild-type

peritoneal inflammation ( J:118171 )

• at 5 months of age, mice show chronic peritonitis

homeostasis/metabolism phenotype ( J:104002 )

N	• adult mice exhibit normal plasma sodium, potassium, bicarbonate and chloride levels relative to wild-type controls

increased circulating creatinine level ( J:104002 )

• adult mice exhibit a 1.5-fold increase in plasma creatinine levels relative to controls

increased blood urea nitrogen level ( J:104002 , J:118171 )

• adult mice exhibit a 2.5-fold increase in plasma BUN levels relative to controls (J:104002)

• BUN levels are increased ~2-fold vs wild-type at 6 months of age (J:118171)

decreased prostaglandin level ( J:109548 )

• near absence of LPS induced synthesis