Analysis Tools
mortality/aging
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• at E10.5, 2 out of 10 double mutants are found dead and 2 are noticeably smaller; also, 9 out of 19 double mutants die at E11.5, that is 2 days earlier than mice homozygous for Rb1tm1Tyj alone
• by E12.5, 7 out of 8 double mutant embryos are either dead or in various stages of resorption
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cellular
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• at E11.5, double mutant embryos exhibit a significantly high frequency of pyknotic nuclei (i.e. apoptosis) in the CNS; in contrast, single Rb1tm1Tyj homozygotes show extensive CNS apoptosis at later stage (E13.5)
• at E12.5 (but not E11.5), a viable double mutant (1 out of 8) exhibits abnormally high levels of apoptosis in the liver and CNS; in contrast, single Rb1tm1Tyj homozygotes show extensive liver apoptosis at later stage
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embryo
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• at E11.5, viable double mutant embryos exhibit disorganization of the endothelial linings of yolk sac vessels
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• at E11.5 days, 2 out of 19 double mutant embryos are significantly smaller than control embryos
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pale yolk sac
(
J:34058
)
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• at E11.5, viable double mutant embryos show a significant reduction of erythrocytes in the yolk sac blood vessels
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growth/size/body
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• at E11.5 days, 2 out of 19 double mutant embryos are significantly smaller than control embryos
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muscle
| N |
• at E12.5, double mutant embryos show normal myogenic commitment and differentiation
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cardiovascular system
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• at E11.5, viable double mutant embryos exhibit disorganization of the endothelial linings of yolk sac vessels
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