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Phenotypes Associated with This Genotype
Genotype
MGI:3576477
Allelic
Composition
Dclre1ctm2Mcow/Dclre1ctm2Mcow
Genetic
Background
involves: 129/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dclre1ctm2Mcow mutation (2 available); any Dclre1c mutation (15 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• thymus showed absence of the lymphocytic cortex, and predominance of connective tissue with scattered lymphoid cells
• thymus weighted approximately 20% of wildtype and was poorly developed at 2 and 4-5 weeks of age
• arrest of B cell development at an early progenitor stage
• more than 90% of thymocytes were arrested at the CD4-CD8- stage, compared to 5-10% arrested in wildtype, there were at least 20 times more CD4-CD8-CD25+ cells and showed near absence of CD8+CD3+ and CD4+CD3+ mature single-positive cells in 2 week old mutants
• lymphoid depletion
• thymoctye counts were 0.7-4% of that observed in wildtype
• spleen was smaller and poorly developed at 2 and 4-5 weeks of age
• spleens showed reduction in fully developed lymphoid follicles, in which majority of lymphoid cells showed larger nuclei with less dense chromatin and prominent nucleoli
• impaired coding joint rearrangement (10 fold decrease in DH-JH2 and 100 fold decrease in VH7183-JH2 rearrangement and 10-100 fold reduction in TCRbeta and Ddelta2-Jdelta1 coding joint formation) while retaining intact signal ends and close to normal signal joint formation
• B cell proliferation to LPS was 13.5% of that in wildtype
• T cell proliferation to Con A and anti-CD3 was reduced to 16.5 and 18.7% of that seen in wildtype
• lymph nodes were severely depleted of mature lymphocytes, with complete absence of lymphoid follicles
• lymph nodes were smaller and poorly developed at 2 and 4-5 weeks of age

hematopoietic system
• thymus showed absence of the lymphocytic cortex, and predominance of connective tissue with scattered lymphoid cells
• thymus weighted approximately 20% of wildtype and was poorly developed at 2 and 4-5 weeks of age
• arrest of B cell development at an early progenitor stage
• more than 90% of thymocytes were arrested at the CD4-CD8- stage, compared to 5-10% arrested in wildtype, there were at least 20 times more CD4-CD8-CD25+ cells and showed near absence of CD8+CD3+ and CD4+CD3+ mature single-positive cells in 2 week old mutants
• lymphoid depletion
• thymoctye counts were 0.7-4% of that observed in wildtype
• spleen was smaller and poorly developed at 2 and 4-5 weeks of age
• spleens showed reduction in fully developed lymphoid follicles, in which majority of lymphoid cells showed larger nuclei with less dense chromatin and prominent nucleoli
• impaired coding joint rearrangement (10 fold decrease in DH-JH2 and 100 fold decrease in VH7183-JH2 rearrangement and 10-100 fold reduction in TCRbeta and Ddelta2-Jdelta1 coding joint formation) while retaining intact signal ends and close to normal signal joint formation
• B cell proliferation to LPS was 13.5% of that in wildtype
• T cell proliferation to Con A and anti-CD3 was reduced to 16.5 and 18.7% of that seen in wildtype

cellular
• coding joint formation was undetectable in MEFs in an extrachromosomal V(D)J recombination assay
• MEFs exhibit elevated sensitivity to ionizing radiation

endocrine/exocrine glands
• thymus showed absence of the lymphocytic cortex, and predominance of connective tissue with scattered lymphoid cells
• thymus weighted approximately 20% of wildtype and was poorly developed at 2 and 4-5 weeks of age
• thymoctye counts were 0.7-4% of that observed in wildtype


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/13/2016
MGI 6.05
The Jackson Laboratory