Analysis Tools
pigmentation
| N |
• homozygotes exhibit normal skin and retinal pigmentation relative to wild-type mice; melanocytes are present at normal numbers in the base of hair follicles
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endocrine/exocrine glands
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• mutant seminiferous tubules contain only two distinct cell types, Sertoli cells and spermatogonial stem cells, in a single outer cell layer
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• mutant males exhibit normal libido and produce vaginal plugs, but their testes are severely hypoplastic relative to wild-type mice
• testicular development proceeds normally until P8, when mutant testes show normal organization and contain the expected cell types, including germ cells, at roughly normal numbers
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growth/size/body
| N |
• homozygotes are viable and healthy and have normal body weights relative to wild-type mice
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hematopoietic system
| N |
• homozygotes display normal hematopoiesis and hematological parameters, including normal white cell and differential counts, as well as normal hemoglobin, mean cell volume and mean cell haemoglobin concentration relative to wild-type mice
• no macrocytic anemia is observed
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reproductive system
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• at P8, male homozygotes exhibit only a few spermatogonial cells relative to wild-type mice
• mutant males display an initial reduction in proliferation and subsequent extensive apoptosis at the spermatogonial stem-cell level, resulting in more undifferentiated spermatogonial stem cells and Sertoli cells that persist through adulthood
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• mutant seminiferous tubules contain only two distinct cell types, Sertoli cells and spermatogonial stem cells, in a single outer cell layer
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• mutant males exhibit normal libido and produce vaginal plugs, but their testes are severely hypoplastic relative to wild-type mice
• testicular development proceeds normally until P8, when mutant testes show normal organization and contain the expected cell types, including germ cells, at roughly normal numbers
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• male homozygotes exhibit arrested spermatogenesis, probably due to an early spermatogonial stem cell differentiation block
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• homozygous mutant males are sterile
• in contrast, mutant females are fully fertile with normal ovaries and a normal number/size of oocytes and ruptured follicles
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cellular
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• at P8, male homozygotes exhibit only a few spermatogonial cells relative to wild-type mice
• mutant males display an initial reduction in proliferation and subsequent extensive apoptosis at the spermatogonial stem-cell level, resulting in more undifferentiated spermatogonial stem cells and Sertoli cells that persist through adulthood
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