Phenotypes Associated with This Genotype

Genotype
MGI:3527983

• Allelic Composition: \Cacna1e^tm1.1Tsch/\Cacna1e^tm1.1Tsch
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

decreased pancreatic alpha cell number ( J:95377 )

• only 7% of dispersed islet cells were alpha cells in mutants compared to 27% of wild-type cells

abnormal glucagon secretion ( J:95377 )

• increased glucose levels did not suppress glucagon secretion as much in mutants as it did in wild-type islets, 27% reduction in mutants compared to a 58% reduction in wild-type islets

• an L-type channel antagonist stimulated glucagon release 52% in the presence of high glucose concentrations in wild-type islets but reduced glucagon secretion by 32% in mutant islets

abnormal pancreatic beta cell physiology ( J:95377 )

• at -10mV the integrated Ca²⁺ currents are reduced about 23% compared to wild-type; this reduction is limited to the high voltage Ca²⁺ current component

• the late component of exocytosis, but not the early component, is suppressed in mutant beta cells

decreased insulin secretion ( J:95377 )

• glucose stimulated insulin release is reduced about 25% in mutant islets compared to wild-type islets

• in situ pancreatic perfusion revealed that the initial peak of insulin secretion is normal however the second-phase of insulin release is reduced by about 46% compared to wild-type

homeostasis/metabolism

abnormal glucagon secretion ( J:95377 )

• increased glucose levels did not suppress glucagon secretion as much in mutants as it did in wild-type islets, 27% reduction in mutants compared to a 58% reduction in wild-type islets

• an L-type channel antagonist stimulated glucagon release 52% in the presence of high glucose concentrations in wild-type islets but reduced glucagon secretion by 32% in mutant islets

decreased insulin secretion ( J:95377 )

• glucose stimulated insulin release is reduced about 25% in mutant islets compared to wild-type islets

• in situ pancreatic perfusion revealed that the initial peak of insulin secretion is normal however the second-phase of insulin release is reduced by about 46% compared to wild-type

increased circulating glucose level ( J:95377 )

• basal glucose levels are elevated by 17% compared to wild-type mice

decreased circulating glucagon level ( J:95377 )

• basal glucagon levels are decreased about 10% compared to wild-type mice and after glucose challenge plasma glucagon levels are increased about 18% in contrast to wild-type mice where after a glucose challenge glucagon levels are decreased by 15%

impaired glucose tolerance ( J:95377 )

• 3 and 8 minutes after a glucose challenge plasma glucose levels are elevated about 15% in mutants compared to wild-type mice