Analysis Tools
homeostasis/metabolism
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• in response to corneal epithelial wounding, homozygotes re-epithelialize debridement wounds at a slower rate (8-hour delay in the 50% wound closure time relative to wild-type)
• in contrast to wild-type, mutant corneal epithelial cells fail to re-stratify properly by 2 weeks and exhibit prolonged hypoplasia
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• in response to corneal epithelial wounding, homozygotes re-epithelialize debridement wounds at a slower rate (8-hour delay in the 50% wound closure time relative to wild-type)
• in contrast to wild-type, mutant corneal epithelial cells fail to re-stratify properly by 2 weeks and exhibit prolonged hypoplasia
• homozygotes show normal cell migration but delayed re-epithelialization of full-thickness cutaneous wounds; hypoplasia of epithelial tissues within the wound bed is observed
• in response to dermabrasion, homozygotes show delayed skin healing at both 2 and 5 days after wounding
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cellular
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• relative to wild-type cells, mutant corneal epithelial cells display a higher cell proliferation rate prior to wounding
• similarly, mutant epidermal keratinocytes proliferate at a significantly higher rate prior to dermabrasion
• wild-type corneal epithelial cells increase proliferation at 24 hours after wounding; in contrast, mutant corneal epithelial cells show reduced localization of alpha9 integrin during closure of wounds and fail to increase their proliferation rate 24 hours after wounding
• at 2 days after dermabrasion, mutant epidermal keratinocytes show only a slight increase in cell proliferation; in wild-type skin, cell proliferation increases >3-fold
• in mutants, extracts from full-thickness skin show increased levels of alpha3 and alpha9 integrins both prior to injury and after hair removal but no increase 2 days after dermabrasion
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immune system
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• after corneal epithelial wounding, homozygotes show a transient increase in inflammatory cells in the corneal stroma
• 18 hours after wounding, more inflammatory cells are found beneath the migrating mutant epithelial sheet than in the wild-type
• in response to dermabrasion, mutant skin wounds appear deeper and tissues more inflamed relative to wild-type
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vision/eye
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• in response to corneal epithelial wounding, homozygotes re-epithelialize debridement wounds at a slower rate (8-hour delay in the 50% wound closure time relative to wild-type)
• in contrast to wild-type, mutant corneal epithelial cells fail to re-stratify properly by 2 weeks and exhibit prolonged hypoplasia
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