Analysis Tools
mortality/aging
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• Background Sensitivity: shorter life span than wild-type in original mixed background
• Background Sensitivity: initial breeding experiments in mixed background generated many live born homozygous pups
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behavior/neurological
head tossing
(
J:63181
)
hearing/vestibular/ear
pigmentation
belly spot
(
J:63181
)
|
• a complete white belt around trunk with some variability
|
nervous system
|
• abnormal neuronal migration contributes to abnormal cerebellum development
|
spina bifida
(
J:63181
)
|
• in vertebrae T9-T13
|
|
• significant changes in the shape of rhombomeres 3, 4, and 5 are observed in homozygote embryos
• however, hindbrain segmentation and territories posterior to r6 are not affected
|
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• r3 is deformed and bends posteriorly
|
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• the dorsal/lateral domains of r4 are compressed or reduced in width
• however, the ventral region of r4 is normal, as shown by proper migration of the facial motor neurone population along the midline into r5
|
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• r5 is broadened and not separated at the midline into two bilateral domains as seen in wild-type embryos
|
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• defective roof plate formation in the hindbrain
|
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• pyramidal layer forms widely dispersed arrangements in this area
• there is aberrant cholinergic activity
|
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• forms widely dispersed arrangements in CA3 region
• there are ectopically located pyramidal cells in the stratum radiatum and stratum oriens
• abnormal acetylcholinesterase activity is found in the ectopic pyramidal cells
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• the cerebellum is almost completely absent
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• disruptions in foliation are found
• there are mixtures of normal and abnormal laminated structure
• islands of both granule and Purkinje cells or just granule cells are observed
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• much reduced glossopharyngeal ganglion
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• an accessory branch originating in the glossopharyngeal/vagus region projects abnormally
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craniofacial
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• the shape and ossification is severely disrupted
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• the shape of the parietal bone is affected
• highly disorganized lamboid suture
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skeleton
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• the shape and ossification is severely disrupted
|
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• the shape of the parietal bone is affected
• highly disorganized lamboid suture
|
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• neural arches in C1 fail to form along the dorsal midline
• delay in fusion of the arches in C2 and C3
• failure in dorsal fusion of vertebrae T9-T13 causing grossly altered neural arches
|
synostosis
(
J:63181
)
|
• occasional synostosis of the cranial vault along the interface of the interparietal and supraoccipital bone
• responsible for the blebbing on the head seen in live animals
|
cellular
|
• an abnormal distribution of acetylcholinesterase is found in the hippocampus
|
|
• abnormal neuronal migration contributes to abnormal cerebellum development
|
|
• disruption of cell proliferation contributes to abnormal cerebellum development
|
embryo
spina bifida
(
J:63181
)
|
• in vertebrae T9-T13
|
|
• significant changes in the shape of rhombomeres 3, 4, and 5 are observed in homozygote embryos
• however, hindbrain segmentation and territories posterior to r6 are not affected
|
|
• r3 is deformed and bends posteriorly
|
|
• the dorsal/lateral domains of r4 are compressed or reduced in width
• however, the ventral region of r4 is normal, as shown by proper migration of the facial motor neurone population along the midline into r5
|
|
• r5 is broadened and not separated at the midline into two bilateral domains as seen in wild-type embryos
|
|
• defective roof plate formation in the hindbrain
|
integument
belly spot
(
J:63181
)
|
• a complete white belt around trunk with some variability
|
