mortality/aging
• all homozygotes are viable up to 11.5 dpc; no embryos are found alive beyond 13.5 dpc
|
cardiovascular system
hemorrhage
(
J:51402
)
• at 11.5 dpc homozygotes are indistinguishable from their wild-type or heterozygous counterparts
• by 12.5 dpc, the majority of mutant embryos display abdominal bleeding
• notably, no hemorrhagic sites are found, and the vasculature of homozygotes appears intact
|
craniofacial
• by 12.5 dpc, most homozygotes display a malformed lower jaw
|
embryo
• by 12.5 dpc, the majority of mutant embryos exhibit retarded growth
|
pale yolk sac
(
J:51402
)
• by 12.5 dpc, the majority of mutant embryos exhibit a pale yolk sac
|
growth/size/body
• by 12.5 dpc, the majority of mutant embryos exhibit retarded growth
|
hematopoietic system
• by 12.5 dpc, the majority of mutant embryos exhibit severe anemia; the amount of peripheral blood is significantly reduced
|
• at 11.5 dpc, homozygotes show a siginificant reduction in fetal liver hematopoiesis
|
• cytospin preparations of blood smears from mutant embryos show only large nucleated red cells of primitive origin, as opposed to the mature non-nucleated red blood cells found in wild-type
|
immune system
N |
• in vitro fetal liver cultures from mutant embryos indicated normal macrophage development
|
liver/biliary system
pale liver
(
J:51402
)
• by 12.5 dpc, mutant livers show a significant reduction in the hematopoietic precursors and an overall pallor
• also, mutant fetal livers exhibit a severe reduction in CFU-E formation, depite normal yolk sac erythropoiesis
|
respiratory system
• in some 11.5 dpc homozygotes, the amount of branching in the lung is slightly reduced
• all other organs appear histologically normal
|
skeleton
• by 12.5 dpc, most homozygotes display a malformed lower jaw
|