Phenotypes Associated with This Genotype

Genotype
MGI:3045815

• Allelic Composition: Alox5^tm1Fun/Alox5^tm1Fun
• Genetic Background: involves: 129S2/SvPas * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

cardiovascular system phenotype ( J:21372 )

N • homozygotes display a normal mean arterial pressure and heart rate relative to wild-type

hematopoietic system

hematopoietic system phenotype ( J:21372 )

N • analysis of cell-surface markers CD3, CD4 and B220 on splenocytes from 4-wk- and 8-wk-old mice revealed no other abnormalities

N • homozygotes show no changes in total blood cell populations or differential cell counts

N • bone marrow analysis revealed no evidence of abnormal precursor cells of any lineage relative to wild-type

small spleen ( J:21372 )

• homozygotes show no abnormalities up to 10 months of age under normal physiological conditions; however, their spleen is usually smaller relative to wild-type

immune system

immune system phenotype ( J:21372 )

N • homozygotes display a normal reaction to endotoxin-induced shock

N • also, homozygotes exhibit a normal reaction to ear inflammation induced by topical application of phorbol myristyl acetate

N • neutrophil recruitment into the peritoneal cavity by a non-specific inflammatory challenge (glycogen) elicits a comparable (large) influx of polymorphonuclear leukocytes in both wild-type and mutant mice

small spleen ( J:21372 )

• homozygotes show no abnormalities up to 10 months of age under normal physiological conditions; however, their spleen is usually smaller relative to wild-type

abnormal immune system physiology ( J:21372 )

• conscious, unanaesthetized homozygotes show resistance to the lethal effects of platelet-activating factor (PAF): they display the same listless behavior as wild-type for the first 20 min but recover quickly

• mechanically ventilated, anesthetized homozygotes show no signs of broncho-constriction; however, broncho-constriction is observed after methacholine challenge

decreased inflammatory response ( J:75371 , J:21372 )

• in the thioglycollate-elicited peritonitis model, homozygotes only generate about 50% as much peritoneal leukocytosis as wild-type mice at 4.5 h after i.p. thioglycollate challenge; as expected, leukotriene B4 is absent from the mutant peritoneal fluid (J:75371)

• the specific arachidonate 5-lipoxygenase inhibitor, zileuton, has no effect on the peripheral white-cell count in mutant mice (J:75371)

• homozygotes show a significantly reduced reaction to arachidonic acid-induced ear inflammation; administration of leukotrienes rescues this inflammatory phenotype: inflammation is rapid in onset and maximal swelling is reached between 30-60 min (J:21372)

• homozygotes display a significantly reduced chemotactic infiltration by polymorphonuclear leukocytes following induction of immune complex peritonitis (reverse passive Arthus reaction) (J:21372)