Phenotypes Associated with This Genotype

Genotype
MGI:3045439

• Allelic Composition: Cxcr4^tm1Tng/Cxcr4^tm1Tng
• Genetic Background: B6.Cg-Cxcr4^tm1Tng

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal T cell differentiation ( J:82996 )

• at E11.5, mutant embryos on a C57BL/6 background showed normal accumulation of T cell precursors in the outer mesenchymal layer of the thymus anlage during initial colonization

• fetal thymi contained relatively normal or slightly reduced numbers of CD3-CD4-CD8- triple-negative (TN) CD44+CD25- and TN CD44+CD25+ cells but substantially reduced numbers of TN CD44-CD25+, TN CD44-CD25-, and CD4+CD8+ double-positive (DP) cells from E13.5 onward

• enforced expression of Bcl2 failed to rescue impaired T cell development

decreased double-positive T cell number ( J:82996 )

• the numbers of CD4+CD8+ DP thymocytes were reduced 5-fold at E18.5

immune system

abnormal T cell differentiation ( J:82996 )

• at E11.5, mutant embryos on a C57BL/6 background showed normal accumulation of T cell precursors in the outer mesenchymal layer of the thymus anlage during initial colonization

• fetal thymi contained relatively normal or slightly reduced numbers of CD3-CD4-CD8- triple-negative (TN) CD44+CD25- and TN CD44+CD25+ cells but substantially reduced numbers of TN CD44-CD25+, TN CD44-CD25-, and CD4+CD8+ double-positive (DP) cells from E13.5 onward

• enforced expression of Bcl2 failed to rescue impaired T cell development

decreased double-positive T cell number ( J:82996 )

• the numbers of CD4+CD8+ DP thymocytes were reduced 5-fold at E18.5

reproductive system

abnormal primordial germ cell migration ( J:83291 )

• from E12.5 to E13.5, mutant embryos on a congenic C57BL/6 background showed a similar expansion of primordial germ cells (PGCs) in the gonads

• at E13.5 or E14.5, the numbers of PGCs in gonads from mutant embryos were significantly reduced, suggesting abnormal homing of PGCs into genital ridges

• in contrast, emigration of PGCs into the endoderm or mesentery of hindgut appeared unaffected

cellular

abnormal primordial germ cell migration ( J:83291 )

• from E12.5 to E13.5, mutant embryos on a congenic C57BL/6 background showed a similar expansion of primordial germ cells (PGCs) in the gonads

• at E13.5 or E14.5, the numbers of PGCs in gonads from mutant embryos were significantly reduced, suggesting abnormal homing of PGCs into genital ridges

• in contrast, emigration of PGCs into the endoderm or mesentery of hindgut appeared unaffected